Testing Data
General Hydroxy Acid Oxidase Assay Kit
Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric)
Reactivity
General
Synonyms
Hydroxy Acid Oxidase; Hydroxy Acid Oxidase Activity Assay Kit (Fluorometric); Hydroxy Acid Oxidase Activity Assay (Fluorometric); Hydroxy Acid Oxidase Activity Assay Kit; HAO Activity Assay Kit; HAO Activity Kit; Hydroxy Acid Oxidase Assay Kit (Fluorometric); Hydroxy Acid Oxidase Activity Assay; Hydroxy Acid Oxidase Activity Kit (Fluorometric); HAO Assay Kit (Fluorometric); HAO Activity Kit (Fluorometric); HAO Activity Assay; HAO Activity Assay Kit (Fluorometric); Hydroxy Acid Oxidase Activity Kit; Hydroxy Acid Oxidase assay kit
Reactivity
General
Samples
Animal tissue lysate (Liver tissue), Plant tissue lysate, Cell lysate, Recombinant enzyme, Purified protein
Detection Method
Fluorescence: Ex/Em 535/587nm
Features & Benefits
The assay can detect as low as 1 uU of HAO activity in biological samples
Simple plate-based fluorometric assay for measuring HAO activity in biological samples.
Simple plate-based fluorometric assay for measuring HAO activity in biological samples.
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Testing Data
Related Product Information for Hydroxy Acid Oxidase assay kit
Hydroxy Acid Oxidase (HAO) (EC 1.1.3.15), also known as glycolate oxidase is a flavin mononucleotide dependent enzyme that oxidizes alpha hydroxy acid to oxo acid. Glycolate is the primary substrate for HAO, which gets oxidized to glyoxalate. HAO contributes to the general pathway of fatty acid alpha-oxidation and plays an important role in ethylene glycol poisoning. It is expressed in liver, kidney and pancreas. The most well studied enzyme of this family is the plant glycolate oxidase that has an essential role in photorespiration. Hydroxy Acid Oxidase Activity Kit is a simple plate-based fluorometric assay for measuring HAO activity in biological samples. HAO oxidizes hydroxy carboxylate, which is accompanied by the release of H?O?. H?O? then oxidizes a non-fluorescent probe to a fluorescent product in the presence of enzyme mix via an enzymatic reaction. The assay can detect as low as 1 uU of HAO activity in biological samples.
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Product Notes
The General Hydroxy Acid Oxidase (Catalog #AAA849585) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849585 Assay Kit recognizes General Hydroxy Acid Oxidase. It is sometimes possible for the material contained within the vial of "Hydroxy Acid Oxidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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