Testing Data
General Acyl-CoA Synthetase Assay Kit
Acyl-CoA Synthetase Fluorometric Assay Kit
Reactivity
General
Synonyms
Acyl-CoA Synthetase; Acyl-CoA Synthetase Fluorometric Assay Kit; Acyl-CoA Synthetase Assay Kit; ACS; Acyl-CoA Synthetase Fluorometric Assay; Acyl-CoA Synthetase Fluorometric Kit; ACS Fluorometric Assay; Acyl-CoA Synthetase Kit; ACS Fluorometric Kit; ACS Fluorometric Assay Kit; Acyl-CoA Synthetase assay kit
Reactivity
General
Samples
Animal tissues: Liver,intestine etc.
Purified enzyme preparations
Cell culture: Adherent or suspension cells
Purified enzyme preparations
Cell culture: Adherent or suspension cells
Detection Method
Fluorescence: Ex/Em 535/587nm
Features & Benefits
To detect ACS activity in various samples
Measurement of Acyl-CoA Synthetase activity in various tissues/cells
Measurement of Acyl-CoA Synthetase activity in various tissues/cells
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Acyl-CoA Synthetase assay kit
Acyl-CoA Synthetase (ACS) also known as Acyl-CoA ligase, is a ubiquitous enzyme that catalyzes the reaction of free fatty acids and Coenzyme A (CoA) to generate Fatty Acyl-CoAs through an ATP-dependent mechanism. The incorporation of this CoA moiety generates a thio-ester bond that effectively activates the fatty acid for downstream biological processes. Unlike their fatty acid counterparts, Fatty acyl-CoAs also serve as precursors of cellular lipids. Dysregulation of the various isoforms of ACS have been implicated in a number of metabolic diseases, including Non-Alcoholic Fatty Liver Disease (NAFLD) and Hepatic Fibrosis. ACS also plays a role in regulating apoptosis. Acyl-CoA Synthetase Fluorometric assay kit provides a rapid, sensitive and straightforward way to measure ACS activity in various samples. In the assay, acyl-CoA produced by ACS activity is metabolized by the Enzyme Mix, Developer Mix and Converter Mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured with excitation at 535 nm and emission at 587 nm. This assay can detect ACS activity as low as 5 mU/ul in samples.
Product Categories/Family for Acyl-CoA Synthetase assay kit
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Product Notes
The General Acyl-CoA Synthetase (Catalog #AAA849569) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849569 Assay Kit recognizes General Acyl-CoA Synthetase. It is sometimes possible for the material contained within the vial of "Acyl-CoA Synthetase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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