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Typical Testing Data/Standard Curve (for reference only)

UreaII Assay Kit

Urea Colorimetric Assay Kit II

Synonyms
UreaII; Urea Colorimetric Assay Kit II; Carbamide Colorimetric Assay Kit II; UreaII assay kit
Ordering
For Research Use Only!
Application Notes
Measurement of urea level in various tissues/cells
Analysis of liver and kidney function
Samples
Biological samples: urine, serum, plasma etc.
Animal tissue: e.g. rat liver, kidney etc.
Adherent or suspension cells: e.g. 3T3 cells, Jurkat cells etc.
Milk
Detection Method
Absorbance (505 nm)
Features & Benefits
Simple procedure; takes ~ 1 hour
Fast and convenient
The kit can measure less than 10 muM urea in 96-well plate assay
Preparation and Storage
4 degree C
Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for UreaII assay kit
Product Information: Urea Colorimetric Assay Kit II: Measures urea level in a variety of samples such as Tissues, Cells, Serum, Plasma, Urine, Milk etc. Convenient & Sensitive. Detection Limit: less than 10 muM. 100 Assays.

Background: Urea is a waste product which is produced in the liver, dissolved and transported in the blood (in a concentration of 1.6-7.5 mM), and excreted by the kidney. Urea plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons. Urea concentration is an important indicator for the medical clinicians to assess function of the kidney and other organs in patients. MyBioSource's Urea Assay Kit II is based on Jung's method with a modification that delivers more robust and sensitive data. The urea is condensed with o-phthalaldehyde (OPA), followed by a reaction to form a colored product with strong absorbance at 505 nm. This assay kit is fast, sensitive & easy to use. It can measure less than 10 muM urea in 96-well plate assay.

Summary: Detection method- Absorbance (505 nm)
Applications: - Measurement of urea level in various tissues/cells - Analysis of liver and kidney function

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Product Notes

The UreaII (Catalog #AAA841721) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of urea level in various tissues/cells Analysis of liver and kidney function. Researchers should empirically determine the suitability of the UreaII for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UreaII, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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