Typical Testing Data/Standard Curve (for reference only)
Xanthine/Hypoxanthine Assay Kit
Xanthine / Hypoxanthine Colorimetric / Fluorometric Assay Kit
Synonyms
Xanthine/Hypoxanthine; Xanthine / Hypoxanthine Colorimetric / Fluorometric Assay Kit; Xanthine/Hypoxanthine assay kit
Samples
Body Fluids: serum, plasma, urine etc.
Animal tissues: liver, muscle, heart etc.
Cell culture: adherent or suspension cells.
Cell and tissue culture supernatant.
Animal tissues: liver, muscle, heart etc.
Cell culture: adherent or suspension cells.
Cell and tissue culture supernatant.
Features & Benefits
Simple procedure; takes ~ 40 minutes
Rapid, sensitive and high-throughput adaptable
Kit contains all necessary reagents for accurate measurement of Xanthine/Hypoxanthine levels
Rapid, sensitive and high-throughput adaptable
Kit contains all necessary reagents for accurate measurement of Xanthine/Hypoxanthine levels
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Xanthine/Hypoxanthine assay kit
Product Information: Xanthine/Hypoxanthine Assay Kit: Rapidly Quantitate Xanthine/Hypoxanthine in serum, plasma, urine, tissues, cells etc. Simple, Sensitive, high-throughput adaptable Colorimetric Assay. Detection limit: 0.4 uM. 100 Assays.
Background: Xanthine, a catabolic product of purine metabolism, is present in body fluids, muscle tissue and certain plants. Structurally like caffeine, Xanthine has a stimulant effect and is used clinically to treat the congestive diseases such as asthma and chronic obstructive pulmonary disease. Xanthine is metabolized into uric acid and superoxide by Xanthine oxidase. Xanthine oxidase deficiency causes the rare genetic disorder-Xanthinuria, and leads to Xanthine accumulation in urine and blood, which ultimately progresses to renal failure. Recent studies show that Xanthine levels are elevated following ischemic injury, thus Xanthine can serve as a useful marker for tissue hypoxia. Early detection of Xanthine alteration in biological fluids is crucial for metabolic studies and for therapeutic monitoring. In MyBioSource's Xanthine/Hypoxanthine Assay kit, Xanthine/Hypoxanthine is specifically oxidized by the Xanthine Enzyme Mix to form an intermediate, which reacts with Developer & Probe to form a product that can be measured colorimetrically (lambda = 570 nm) or fluorometrically (Ex/Em = 535/587 nm). Xanthine/Hypoxanthine Assay kit is rapid, simple and sensitive. This high-throughput suitable assay kit can detect Xanthine levels as low as 0.4 uM in various biological samples.
Summary: Detection method- Absorbance (570 nm) or Fluoroescence (Ex/Em = 535/587 nm)
Application- The kit can detect Xanthine/Hypoxanthine levels as low as 0.4 uM in various biological samples.
Background: Xanthine, a catabolic product of purine metabolism, is present in body fluids, muscle tissue and certain plants. Structurally like caffeine, Xanthine has a stimulant effect and is used clinically to treat the congestive diseases such as asthma and chronic obstructive pulmonary disease. Xanthine is metabolized into uric acid and superoxide by Xanthine oxidase. Xanthine oxidase deficiency causes the rare genetic disorder-Xanthinuria, and leads to Xanthine accumulation in urine and blood, which ultimately progresses to renal failure. Recent studies show that Xanthine levels are elevated following ischemic injury, thus Xanthine can serve as a useful marker for tissue hypoxia. Early detection of Xanthine alteration in biological fluids is crucial for metabolic studies and for therapeutic monitoring. In MyBioSource's Xanthine/Hypoxanthine Assay kit, Xanthine/Hypoxanthine is specifically oxidized by the Xanthine Enzyme Mix to form an intermediate, which reacts with Developer & Probe to form a product that can be measured colorimetrically (lambda = 570 nm) or fluorometrically (Ex/Em = 535/587 nm). Xanthine/Hypoxanthine Assay kit is rapid, simple and sensitive. This high-throughput suitable assay kit can detect Xanthine levels as low as 0.4 uM in various biological samples.
Summary: Detection method- Absorbance (570 nm) or Fluoroescence (Ex/Em = 535/587 nm)
Application- The kit can detect Xanthine/Hypoxanthine levels as low as 0.4 uM in various biological samples.
Product Categories/Family for Xanthine/Hypoxanthine assay kit
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Product Notes
The Xanthine/Hypoxanthine (Catalog #AAA841577) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Xanthine/Hypoxanthine, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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