Cell culture - Adherent or suspension cells
Enzymatic reactions
Simple, fast and convenient.
This assay can measure less than 8 nM NADH in a variety of samples.
Shelf Life: 12 months
Background: NAD and NADH exist in all living organisms and are important for energy transformation and maintenance of the redox state of cells or tissues. The reduced form, NADH is a driving force for cells and plays a critical role in cell regulation and repair processes. It is of increasing interest to be able to measure low level of NADH in samples or in enzymatic reactions. MyBioSource's PicoProbe NADH Assay kit provides a highly sensitive method to detect low level of NADH (NAD is not detected in the reaction). There is no requirement to purify NADH from samples. The NADH Recycling Enzyme Mix specifically recognizes NADH (not NADPH) in the enzyme recycling reaction. The assay is rapid, simple and can measure less than 8 nM NADH in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em=535/587 nm)
Applications: -Measurement of NADH in various tissues/cells -Measurement of low levels of NADH in enzymatic reactions -Analysis of metabolism and cell signaling in various cells
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Product Notes
The PicoProbe NADH (Catalog #AAA841462) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PicoProbe NADH, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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