Typical Testing Data/Standard Curve (for reference only)
Citrate Assay Kit
Citrate Colorimetric / Fluorometric Assay Kit
Synonyms
Citrate; Citrate Colorimetric / Fluorometric Assay Kit; Citrate assay kit
Samples
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes ~ 40 minutes
Fast and convenient
Kit contains all necessary reagents for accurate measurement of citrate levels
Fast and convenient
Kit contains all necessary reagents for accurate measurement of citrate levels
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Citrate assay kit
Product Information: Citrate Assay Kit: Colorimetric & Fluorometric Assay for detecting Citrate in variety of Samples within 40 min. Rapid, Convenient & Reliable. Detection Range: 0.1 to 10 nmoles (~2 muM-10 mM). 100 Assays.
Background: Citric acid (HOOC-CH?-C(-OH)(-COOH)-CH?-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho- fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors. MyBioSource's Citrate Assay Kit provides a simple, sensitive and rapid means of quantifying citrate in a variety of samples. In the assay, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product. The Citrate Assay Kit can detect 0.1 to 10 nmoles (~2 uM-10 mM) of citrate in a variety of samples.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The Citrate Assay Kit can detect 0.1 to 10 nmoles (~2 uM-10 mM) of citrate in a variety of samples.
Background: Citric acid (HOOC-CH?-C(-OH)(-COOH)-CH?-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho- fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors. MyBioSource's Citrate Assay Kit provides a simple, sensitive and rapid means of quantifying citrate in a variety of samples. In the assay, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product. The Citrate Assay Kit can detect 0.1 to 10 nmoles (~2 uM-10 mM) of citrate in a variety of samples.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The Citrate Assay Kit can detect 0.1 to 10 nmoles (~2 uM-10 mM) of citrate in a variety of samples.
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Product Notes
The Citrate (Catalog #AAA841898) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Citrate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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