Typical Testing Data/Standard Curve (for reference only)
Adipogenesis Assay Kit
Adipogenesis Colorimetric / Fluorometric Assay Kit
Synonyms
Adipogenesis; Adipogenesis Colorimetric / Fluorometric Assay Kit; Adipogenesis assay kit
Samples
Adipocyte precursors such as 3T3 cells, human preadipocytes etc.
Features & Benefits
Simple procedure; takes ~40 minutes
Fast and convenient
Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipogenesis in various samples.
Fast and convenient
Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipogenesis in various samples.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Adipogenesis assay kit
Product Information: Adipogenesis Assay Kit: Colorimetric & Fluorometric Assay for Quantifying Triglyceride Accumulation in Cells and Tissues within 40 min. Highly Sensitive, Convenient & Simple. Detection Range: 0.2 to 10 nmol.100 Assays
Background: Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured. MyBioSource's adipogenesis assay kit quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (lambdamax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10 nmol. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or inhibitors, or to screen drugs.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Application- The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10 nmol.
Background: Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured. MyBioSource's adipogenesis assay kit quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (lambdamax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10 nmol. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or inhibitors, or to screen drugs.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Application- The kit can detect triglyceride in as few as a thousand or less differentiated 3T3-L1 cells with triglyceride detection linear range 0.2 to 10 nmol.
Product Categories/Family for Adipogenesis assay kit
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Product Notes
The Adipogenesis (Catalog #AAA841527) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Adipogenesis, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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