Typical Testing Data/Standard Curve (for reference only)
Oxaloacetate Assay Kit
Oxaloacetate Colorimetric / Fluorometric Assay Kit
Synonyms
Oxaloacetate; Oxaloacetate Colorimetric / Fluorometric Assay Kit; Oxaloacetate assay kit
Samples
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes ~ 40 minutes
Fast and convenient
Kit contains all necessary reagents for accurate measurement of OAA levels
Fast and convenient
Kit contains all necessary reagents for accurate measurement of OAA levels
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Oxaloacetate assay kit
Product Information: Oxaloacetate Assay Kit: Colorimetric & Fluorometric Assay for Quantifying Oxaloacetate (OAA) in a variety of Samples such as Tissues, Cells etc. within 40 min. Rapid, Convenient & Reliable. Detection Range: 0.1-10nmol (2-200 muM). 100 Assays.
Background: Oxaloacetate (OAA, HOOC-CO-CH?-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. MyBioSource's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (lambdamax= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 uM) of OAA.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 uM) of OAA.
Background: Oxaloacetate (OAA, HOOC-CO-CH?-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. MyBioSource's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (lambdamax= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 uM) of OAA.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 uM) of OAA.
Product Categories/Family for Oxaloacetate assay kit
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Product Notes
The Oxaloacetate (Catalog #AAA841520) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Oxaloacetate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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