Testing Data
Eukaryotes Nitrite Assay Kit
Nitrite Assay Kit (Griess Reagent)
Reactivity
Eukaryotes
Synonyms
Nitrite; Nitrite Assay Kit (Griess Reagent); Nitrite assay kit
Reactivity
Eukaryotes
Samples
Animal tissues: liver, kidney, etc.
Serum, plasma,urine
Cell culture: Adherent or suspension cells
Serum, plasma,urine
Cell culture: Adherent or suspension cells
Detection Method
Absorbance: 540nm
Features & Benefits
Simple two-step procedure; takes only ~1-1.5 hours
Fast and convenient
Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of total nitrite.
Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.
Detects total nitrite
Fast and convenient
Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of total nitrite.
Nitrite Assay Kit using Griess Reagent provides an accurate, convenient measure of nitrite in a simple two-step process.
Detects total nitrite
Preparation and Storage
Store at 4 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Nitrite assay kit
Nitrogen-based ions, nitrite and nitrate, are found in almost every living organism. Furthermore, endogenous Nitrite levels are found in mammals and also can be obtained from dietary sources. In humans, nitrite is further metabolized to Nitric Oxide and other reactive nitrogen species (nitrogen oxides). The Nitrate-Nitrite-NO biochemical pathway is well known for its participation in cell signaling, hypoxia-dependent response and regulation of blood flow. Recent studies suggest the reduction of Nitrite to Nitrogen Oxygen in the mitochondria. Specifically, myoglobin and xanthine oxidoreductase could generate NO under hypoxic conditions leading to mitochondrial respiration. Nitrite Assay Kit utilizes the Griess Reagent, a classic protocol for the estimation of nitrite. In the assay, nitrite is reduced to Nitrogen Oxide using Griess Reagent I. Then, Nitrogen Oxide reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at OD 540 nm. The one-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.
Product Categories/Family for Nitrite assay kit
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Product Notes
The Eukaryotes Nitrite (Catalog #AAA849728) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849728 Assay Kit recognizes Eukaryotes Nitrite. It is sometimes possible for the material contained within the vial of "Nitrite, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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