Typical Testing Data/Standard Curve (for reference only)
Triose Phosphate Isomerase (TPI) Assay Kit | Tpi1 assay kit
Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit
Gene Names
Tpi1; Tpi; Tpi-1; AI255506
Synonyms
Triose Phosphate Isomerase (TPI); Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit; Tpi1 assay kit
Sequence Length
299
Application Notes
Measurement of TPI activity in various tissues and cells
Analysis of glycolysis & pentose phosphate pathway and lipid metabolism
Analysis of glycolysis & pentose phosphate pathway and lipid metabolism
Samples
Animal tissues: muscle, liver, heart, kidney, etc.
Cell culture: Adherent or suspension Cells
Human serum or plasma
Cell culture: Adherent or suspension Cells
Human serum or plasma
Detection Method
Absorbance (OD 450 nm)
Features & Benefits
Simple, highly sensitive, and high-throughput.
The assay can measure Triose Phosphate Isomerase activity as low as 40 mU/ml.
The assay can measure Triose Phosphate Isomerase activity as low as 40 mU/ml.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Tpi1 assay kit
Product Information: Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit: Only kit on the market to measure TPI activity. Simple, sensitive & high-throughput. Sample type: tissues, cells & serum or plasma. Detection limit: as low as 40 mU/ml.100 Assays
Background: Triose Phosphate Isomerase (TPI or TIM: EC 5.3.1.1) is an important enzyme for glycolysis. It reversibly interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms. In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment. MyBioSource's Triose Phosphate Isomerase Activity Assay kit provides a quick and easy way for monitoring Triose Phosphate Isomerase activity in a variety of samples. In this kit, Triose Phosphate Isomerase converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the Enzyme Mix & Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, & high-throughput and can detect Triose Phosphate Isomerase activity as low as 40 mU/ml.
Summary: Detection method- Absorbance (OD 450 nm)
Applications: - Measurement of TPI activity in various tissues and cells - Analysis of glycolysis & pentose phosphate pathway and lipid metabolism
Background: Triose Phosphate Isomerase (TPI or TIM: EC 5.3.1.1) is an important enzyme for glycolysis. It reversibly interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms. In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment. MyBioSource's Triose Phosphate Isomerase Activity Assay kit provides a quick and easy way for monitoring Triose Phosphate Isomerase activity in a variety of samples. In this kit, Triose Phosphate Isomerase converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the Enzyme Mix & Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, & high-throughput and can detect Triose Phosphate Isomerase activity as low as 40 mU/ml.
Summary: Detection method- Absorbance (OD 450 nm)
Applications: - Measurement of TPI activity in various tissues and cells - Analysis of glycolysis & pentose phosphate pathway and lipid metabolism
Product Categories/Family for Tpi1 assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,192 Da
NCBI Official Full Name
triosephosphate isomerase
NCBI Official Synonym Full Names
triosephosphate isomerase 1
NCBI Official Symbol
Tpi1
NCBI Official Synonym Symbols
Tpi; Tpi-1; AI255506
NCBI Protein Information
triosephosphate isomerase
UniProt Protein Name
Triosephosphate isomerase
UniProt Gene Name
Tpi1
UniProt Synonym Gene Names
Tpi; TIM
UniProt Entry Name
TPIS_MOUSE
Uniprot Description
TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.
Protein type: Carbohydrate Metabolism - fructose and mannose; EC 5.3.1.1; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - inositol phosphate
Cellular Component: extracellular space; nucleus
Molecular Function: isomerase activity; catalytic activity; triose-phosphate isomerase activity
Biological Process: pentose-phosphate shunt; embryonic development; glycolysis; metabolic process; multicellular organismal development; glucose metabolic process; glyceraldehyde-3-phosphate metabolic process; gluconeogenesis
Research Articles on Tpi1
Similar Products
Product Notes
The Tpi1 tpi1 (Catalog #AAA841798) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of TPI activity in various tissues and cells Analysis of glycolysis & pentose phosphate pathway and lipid metabolism. Researchers should empirically determine the suitability of the Tpi1 tpi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Triose Phosphate Isomerase (TPI), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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