Typical Testing Data/Standard Curve (for reference only)
Enolase Assay Kit | ENO assay kit
Enolase Activity Colorimetric / Fluorometric Assay Kit
Synonyms
Enolase; Enolase Activity Colorimetric / Fluorometric Assay Kit; 2-phospho-D-glycerate hydrolase Activity Colorimetric/Fluorometric Assay Kit; 2-phosphoglycerate dehydratase Activity Colorimetric/Fluorometric Assay Kit; ENO assay kit
Sequence Length
433
Application Notes
Measurement of enolase activity in various tissues/cells
Analysis of glycolytic metabolism in different tissues/cells
Mechanistic studies of various cancers
Screening anti-cancer drugs
Analysis of glycolytic metabolism in different tissues/cells
Mechanistic studies of various cancers
Screening anti-cancer drugs
Samples
Animal tissues: Liver, muscle, heart, kidney etc.
Cell culture: adherent or suspension cells
Cell culture: adherent or suspension cells
Detection Method
Absorbance (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm)
Features & Benefits
Rapid and easy-to-use.
Highly-sensitive.
This assay kit can measure less than 0.04 mU of enolase activity in a variety of samples.
Highly-sensitive.
This assay kit can measure less than 0.04 mU of enolase activity in a variety of samples.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ENO assay kit
Product Information: Enolase Activity Colorimetric/Fluorometric Assay Kit: Only kit on the market to measure enolase activity. Simple & rapid. Detection limit: less than 0.04 mU. 100 assays.
Background: Enolase (EC 4.2.1.11), also called 2-phospho-D-glycerate hydrolase or 2-phosphoglycerate dehydratase, is a key enzyme in glycolysis. It converts 2-phosphoglycerate to phosphoenolpyruvate (PEP) & also catalyzes the reverse reaction, PEP to 2-phosphoglycerate under anabolic conditions during gluconeogenesis. In mammals, Enolase consists of three subunits: alpha, beta and gamma that combine into 5 isozymes, 3 of which are commonly found in human tissues. This enzyme exists in all organisms, which can undergo glycolysis. Besides its role in glycolysis, enolase has other functions too. Recent studies have shown that increased activity of enolase is associated with tumor invasion and metastasis; therefore, precise measurement of enolase activity may be of great interest for tumor identification and prognosis. In MyBioSource's Enolase Activity Assay Kit, enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.
Summary: Detection method- Absorbance (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm)
Applications: - Measurement of enolase activity in various tissues/cells - Analysis of glycolytic metabolism in different tissues/cells - Mechanistic studies of various cancers - Screening anti-cancer drugs
Background: Enolase (EC 4.2.1.11), also called 2-phospho-D-glycerate hydrolase or 2-phosphoglycerate dehydratase, is a key enzyme in glycolysis. It converts 2-phosphoglycerate to phosphoenolpyruvate (PEP) & also catalyzes the reverse reaction, PEP to 2-phosphoglycerate under anabolic conditions during gluconeogenesis. In mammals, Enolase consists of three subunits: alpha, beta and gamma that combine into 5 isozymes, 3 of which are commonly found in human tissues. This enzyme exists in all organisms, which can undergo glycolysis. Besides its role in glycolysis, enolase has other functions too. Recent studies have shown that increased activity of enolase is associated with tumor invasion and metastasis; therefore, precise measurement of enolase activity may be of great interest for tumor identification and prognosis. In MyBioSource's Enolase Activity Assay Kit, enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.
Summary: Detection method- Absorbance (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm)
Applications: - Measurement of enolase activity in various tissues/cells - Analysis of glycolytic metabolism in different tissues/cells - Mechanistic studies of various cancers - Screening anti-cancer drugs
Product Categories/Family for ENO assay kit
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Enolase eno (Catalog #AAA841683) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of enolase activity in various tissues/cells Analysis of glycolytic metabolism in different tissues/cells Mechanistic studies of various cancers Screening anti-cancer drugs. Researchers should empirically determine the suitability of the Enolase eno for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Enolase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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