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100 results for " Cytotoxicity Assays" - showing 1-50
alpha-Gal Epitope, Monoclonal Antibody (Cat# AAA603355)
Full Name
alpha-Gal Epitope (Galalpha1-3Galbeta1-4GlcNAc-R)
Applications
EL/EIA, WB, IHC, FC/FACS, can be used for cytotoxicity assays for alpha-Gal specific pathways
Purity
Supernatant
Pricing
$805/1 Each | $3,605/5x1 Each
Testing Data
(ICT's TotalCytotoxicity Testcan be usedto easily analyzethe activity ofnatural killer(NK) cells. Basedon flowcytometric data,the formula[R2/(R2+R1)] X 100 was used tocalculate cytotoxicity. NK-killed necrotic cells are shown in the maroon line, andapoptotic cells are shown as the blue line. When added together, the total levelof cell death is calculated as the black line. In this example, cytotoxicityincreased as more NK effector cells were combined with target cells. As the ratioof Effector:Target-cells increased from 12.5:1 to 100:1 the percentage of targetcells dying increased from 11% to 60%.)
Cytotoxicity, Assay Kit (Cat# AAA258006)
Full Name
Total Cytotoxicity Test Assay Kit
Pricing
$715/250 Tests | $435/125 Tests | $3,235/5x250 Tests
Immunofluorescence (IF)
( Immunofluorescence staining of fixed U937 cells with anti-CD64 antibody H22 Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of H22 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD64, Monoclonal Recombinant Antibody (Cat# AAA488344)
Full Name
Anti-CD64 [H22]
Gene Names
FCGR1A; CD64; FCRI; CD64A; IGFR1
Reactivity
Human
Applications
EIA, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Fc gamma receptor III antibody 3G8 (Ab00754) Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of 3G8 (Ab00754-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Fc gamma receptor III, Monoclonal Recombinant Antibody (Cat# AAA488350)
Full Name
Anti-Fc gamma receptor III [3G8]
Reactivity
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset
Applications
FC/FACS, SPR, IP, IHC, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Activity Data
(Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1,is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It can also affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy. Besides, Colony Stimulating Factor Receptor, Macrophage (M-CFS-R) has been identified as an interactor of M-CSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse M-CSF and recombinant mouse MCFSR Briefly, M-CSF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to M-CFS-R-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-M-CSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of M-CSF and M-CFS-R was shown in Figure 1, and this effect was in a dose dependent manner.)
Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein (Cat# AAA2097263)
Full Name
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Gene Names
Csf1; op; Csfm; MCSF; C87615
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$205/0.01 mg | $415/0.05 mg | $635/0.1 mg | $790/0.2 mg | $1,530/0.5 mg | $2,280/1 mg | $5,565/5 mg
Testing Data
(Published customer image: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 ug/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p)
CD335, Monoclonal Antibody (Cat# AAA211240)
Full Name
MOUSE ANTI BOVINE CD335:Low Endotoxin
Gene Names
NCR1; NKP46
Applications
FC/FACS, FN, IF, WB
Pricing
$755/0.5 mg | $3,255/5x0.5 mg
CD64, Monoclonal Recombinant Antibody (Cat# AAA488343)
Full Name
Anti-CD64 [H22]
Gene Names
FCGR1A; CD64; FCRI; CD64A; IGFR1
Reactivity
Human
Applications
EIA, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Tumor Necrosis Factor Receptor 2, Active Protein (Cat# AAA650326)
Full Name
Tumor Necrosis Factor Receptor 2, Recombinant, Human (TNFR2, CD120b)
Gene Names
TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Purity
Highly Purified
98% (SDS PAGE and HPLC).
98% (SDS PAGE and HPLC).
Pricing
$670/0.02 mg | $3,005/5x0.02 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Caco-2 cells with anti-LeY tetrasaccharide antibody BR96 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of BR96 (MBS488427) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488427, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
LeY, Monoclonal Recombinant Antibody (Cat# AAA488427)
Full Name
Anti-LeY [BR96]
Reactivity
Human
Applications
EIA, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Caco-2 cells with anti-LeY tetrasaccharide antibody BR96 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of BR96 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
LeY, Monoclonal Recombinant Antibody (Cat# AAA488428)
Full Name
Anti-LeY [BR96]
Reactivity
Human
Applications
EIA, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD52, Recombinant Antibody (Cat# AAA488134)
Full Name
Anti-CD52 [YTH 34.5-G2b (Campath-1G)]
Reactivity
Human, Rhesus Monkey
Applications
EIA, IHC, FC/FACS, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-CCR4 antibody KW-0761 (Mogamulizumab) Human brain cerebellum (A) and human heart (B) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of KW-0761 (MBS488458) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CCR4 is 37.7kDa.)
CCR4, Monoclonal Recombinant Antibody (Cat# AAA488458)
Full Name
Anti-CCR4 [KW-0761 (Mogamulizumab)]
Gene Names
CCR4; CKR4; K5-5; CD194; CMKBR4; ChemR13; CC-CKR-4; HGCN:14099
Reactivity
Human
Applications
Deplete
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
CD52, Recombinant Antibody (Cat# AAA488133)
Full Name
Anti-CD52 [YTH 34.5-G2b (Campath-1G)]
Gene Names
CD52; CDW52
Reactivity
Human, Rhesus Monkey
Applications
EIA, IHC, FC/FACS, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
CytoSelect LDH Cytotoxicity, Assay Kit (Cat# AAA168668)
Full Name
CytoSelect LDH Cytotoxicity Assay Kit
Pricing
$455/960 Assays | $2,070/5x960 Assays
Testing Data
(ICT's Basic Cytotoxicity Testcan beused to easily analyze theactivity of natural killer (NK)cells. Based on flowcytometric data, theformula [R2/(R2+R1)] X 100was used to calculatecytotoxicity. In this example,cytotoxicity increased as moreNK effector cells were combinedwith target cells. As the ratio ofEffector:Target-cells increasedfrom 12.5:1 to 100:1 the percentage of target cells dying increased from 10% to40%.)
Cytotoxicity, Assay Kit (Cat# AAA258005)
Full Name
Basic Cytotoxicity Test Assay Kit
Pricing
$360/250 Tests | $265/125 Tests | $1,585/5x250 Tests
MHC Class II, Monoclonal Antibody (Cat# AAA673637)
Full Name
Rat Anti-Mouse MHC Class II-UNLB
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Reactivity
Mouse
Applications
Flow Cytometry
Pricing
$325/0.5 mg | $1,310/5x0.5 mg
Testing Data
HLA-ABC, Monoclonal Antibody (Cat# AAA520339)
Full Name
Anti-Human HLA-ABC, Purified, (Clone W6/32 HLK) (mouse IgG2a)
Reactivity
Human
Applications
IHC, CA, IP
Pricing
$395/0.5 mg | $1,555/5x0.5 mg
PMN, Polyclonal Antibody (Cat# AAA524078)
Full Name
Anti-Mouse PMN, Antiserum, (Polyclonal) (rabbit serum)
Reactivity
Mouse
Applications
CA, IV
Pricing
$560/5 mL | $2,300/5x5 mL
Testing Data
Lymphocytes, Antibody (Cat# AAA524030)
Full Name
Rabbit Anti-Mouse Lymphocyte Serum
Reactivity
Mouse
Pricing
$410/5 mL | $1,630/5x5 mL
Testing Data #2
(alamarBlueR indicator dye (MBS238968) quantitatively measures proliferation in human, animal, bacterial, fungal and mycobacterial cells. It is useful for cytokine bioassays, cell proliferation studies and in-vitro cytotoxicity determinations as well as cell growth monitoring)
alamarBlue, Accessory Reagent (Cat# AAA238967)
Full Name
alamarBlue
Applications
EIA, FN, IF
Pricing
$280/25 mL | $1,100/5x25 mL
Testing Data
CD90 (Thy 1.1), Monoclonal Antibody (Cat# AAA520243)
Full Name
Anti-Mouse CD90 (Thy 1.1), Supernatant (Clone T11D7e) (mouse IgM)
Reactivity
Mouse
Applications
CA
Pricing
$430/5 mL | $1,720/5x5 mL
Testing Data
I-Ed (private; Ia.m23), Monoclonal Antibody (Cat# AAA520369)
Full Name
Anti-Mouse I-Ed (private; Ia.m23), Ascites (Clone 34-1-45) (mouse IgM)
Pricing
$530/1 mL | $2,170/5x1 mL
Testing Data
H-2KbDb (private), Monoclonal Antibody (Cat# AAA520327)
Full Name
Anti-Mouse H-2KbDb (private), Purified (Clone 5041.16.1) (mouse IgG2a)
Reactivity
Mouse
Applications
IHC, CA
Purity
Purified from ascitic fluid via protein G affinity chromatography.
Pricing
$320/0.25 mg | $1,220/5x0.25 mg
Testing Data
MHC Class II, Monoclonal Antibody (Cat# AAA673638)
Full Name
Rat Anti-Mouse MHC Class II-FITC
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Reactivity
Mouse
Applications
Flow Cytometry
Pricing
$370/0.5 mg | $1,520/5x0.5 mg
MHC Class II, Monoclonal Antibody (Cat# AAA673640)
Full Name
Rat Anti-Mouse MHC Class II-PE
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Reactivity
Mouse
Applications
Flow Cytometry
Pricing
$285/0.1 mg | $1,145/5x0.1 mg
MHC Class II, Monoclonal Antibody (Cat# AAA673643)
Full Name
Rat Anti-Mouse MHC Class II-LE/AF
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Reactivity
Mouse
Applications
Flow Cytometry
Pricing
$420/0.5 mg | $1,745/5x0.5 mg
MHC Class II, Monoclonal Antibody (Cat# AAA673639)
Full Name
Rat Anti-Mouse MHC Class II-BIOT
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Reactivity
Mouse
Applications
Flow Cytometry
Pricing
$345/0.5 mg | $1,405/5x0.5 mg
CD8a, Monoclonal Antibody (Cat# AAA673820)
Full Name
Mouse Anti-Porcine CD8a-SPRD
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Porcine
Applications
Flow Cytometry; Immunoprecipitation
Pricing
$315/0.1 mg | $1,275/5x0.1 mg
MHC Class II, Monoclonal Antibody (Cat# AAA673641)
Full Name
Rat Anti-Mouse MHC Class II-PE
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Reactivity
Mouse
Applications
Flow Cytometry
Pricing
$395/0.2 mg | $1,620/5x0.2 mg
NK1.1 (Ly-55), Monoclonal Antibody (Cat# AAA673559)
Full Name
Mouse Anti-Mouse NK1.1-UNLB
Reactivity
Mouse
Applications
Flow Cytometry; Immunoprecipitation
Pricing
$285/0.5 mg | $1,130/5x0.5 mg
Testing Data
HLA-ABC, Monoclonal Antibody (Cat# AAA520455)
Full Name
Anti-Human HLA-ABC, PE, (Clone W6/32 HLK) (mouse IgG2a)
Reactivity
Human
Applications
IHC
Pricing
$295/0.05 mg | $1,100/5x0.05 mg
MHC Class II, Monoclonal Antibody (Cat# AAA673642)
Full Name
Rat Anti-Mouse MHC Class II-SPRD
Gene Names
HLA-DQB1; IDDM1; CELIAC1; HLA-DQB
Reactivity
Mouse
Applications
Flow Cytometry
Pricing
$355/0.1 mg | $1,440/5x0.1 mg
Ly-6G (GR-1), Monoclonal Secondary Antibody (Cat# AAA673657)
Full Name
Rat Anti-Mouse Ly-6G/GR-1-UNLB
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$255/0.5 mg | $990/5x0.5 mg
NK1.1 (Ly-55), Monoclonal Antibody (Cat# AAA673563)
Full Name
Mouse Anti-Mouse NK1.1-PE
Reactivity
Mouse
Applications
Flow Cytometry; Immunoprecipitation
Pricing
$335/0.2 mg | $1,370/5x0.2 mg
NK1.1 (Ly-55), Monoclonal Antibody (Cat# AAA673560)
Full Name
Mouse Anti-Mouse NK1.1-FITC
Reactivity
Mouse
Applications
Flow Cytometry; Immunoprecipitation
Pricing
$335/0.5 mg | $1,370/5x0.5 mg
NK1.1 (Ly-55), Monoclonal Antibody (Cat# AAA673564)
Full Name
Mouse Anti-Mouse NK1.1-APC
Reactivity
Mouse
Applications
Flow Cytometry; Immunoprecipitation
Pricing
$350/0.1 mg | $1,420/5x0.1 mg
Testing Data
NK1.1 (Ly-55), Monoclonal Antibody (Cat# AAA673562)
Full Name
Mouse Anti-Mouse NK1.1-PE
Reactivity
Mouse
Applications
Flow Cytometry; Immunoprecipitation
Pricing
$265/0.1 mg | $1,030/5x0.1 mg
NK1.1 (Ly-55), Monoclonal Antibody (Cat# AAA673565)
Full Name
Mouse Anti-Mouse NK1.1-LE/AF
Reactivity
Mouse
Applications
Flow Cytometry; Immunoprecipitation
Pricing
$345/0.5 mg | $1,405/5x0.5 mg
NK1.1 (Ly-55), Monoclonal Antibody (Cat# AAA673566)
Full Name
Mouse Anti-Mouse NK1.1-PE/CY7
Reactivity
Mouse
Applications
Flow Cytometry; Immunoprecipitation
Pricing
$275/0.1 mg | $1,085/5x0.1 mg
Ly-6G (GR-1), Monoclonal Secondary Antibody (Cat# AAA673667)
Full Name
Rat Anti-Mouse Ly-6G/GR-1-PE/CY7
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$275/0.1 mg | $1,085/5x0.1 mg
Ly-6G (GR-1), Monoclonal Secondary Antibody (Cat# AAA673668)
Full Name
Rat Anti-Mouse Ly-6G/GR-1-APC/CY7
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$315/0.1 mg | $1,260/5x0.1 mg
Ly-6G (GR-1), Monoclonal Secondary Antibody (Cat# AAA673663)
Full Name
Rat Anti-Mouse Ly-6G/GR-1-SPRD
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$290/0.1 mg | $1,155/5x0.1 mg
TNF ALPHA, Monoclonal Antibody (Cat# AAA210239)
Full Name
MOUSE ANTI PIG TNF ALPHA:Preservative Free
Gene Names
TNF; TNFa
Applications
EIA, FN, WB
Pricing
$655/0.5 mg | $2,800/5x0.5 mg
Ly-6G (GR-1), Monoclonal Secondary Antibody (Cat# AAA673660)
Full Name
Rat Anti-Mouse Ly-6G/GR-1-PE
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$260/0.1 mg | $1,015/5x0.1 mg
Ly-6G (GR-1), Monoclonal Secondary Antibody (Cat# AAA673662)
Full Name
Rat Anti-Mouse Ly-6G/GR-1-APC
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$260/0.1 mg | $1,025/5x0.1 mg
Ly-6G (GR-1), Monoclonal Secondary Antibody (Cat# AAA673664)
Full Name
Rat Anti-Mouse Ly-6G/GR-1-LE/AF
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$315/0.5 mg | $1,275/5x0.5 mg
Macrophages, Polyclonal Antibody (Cat# AAA524051)
Full Name
Anti-Rat Macrophages, Adsorbed Antiserum, (Polyclonal) (rabbit serum)
Applications
CA, IHC
Pricing
$410/2 mL | $1,630/5x2 mL
Testing Data
Ly-6G (GR-1), Monoclonal Antibody (Cat# AAA673658)
Full Name
Rat Anti-Mouse Ly-6G/GR-1-FITC
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$315/0.5 mg | $1,260/5x0.5 mg
LDH, Polyclonal Antibody (Cat# AAA534030)
Full Name
LDH antibody
Reactivity
Pig
Applications
User optimized
Pricing
$185/1 mL | $835/5x1 mL
Ly-6G (GR-1), Monoclonal Secondary Antibody (Cat# AAA673665)
Full Name
Rat Anti-Mouse Ly-6G/GR-1-CY5
Reactivity
Mouse
Applications
Frozen Sections
Pricing
$340/0.1 mg | $1,385/5x0.1 mg
