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GFR Alpha-3/GFRA3 Active Protein | GFRA3 active protein
GFR Alpha-3/GFRA3 Protein, Human, Recombinant (His & hFc Tag)
Gene Names
GFRA3; GDNFR3
Purity
>80% as determined by SDS-PAGE
Synonyms
GFR Alpha-3/GFRA3; GFR Alpha-3/GFRA3 Protein; Human; Recombinant (His & hFc Tag); Human GFRA3/GFR-alpha-3 Protein (His & Fc Tag); GDNFR3 Protein; GDNF family receptor alpha 3; GFRA3 active protein
Host
HEK293 Cells
Purity/Purification
>80% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Trp382
Species
Human
Activity
Measured by its ability to bind mouse ARTN in a functional ELISA.
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Asp 32
Tag
C-human IgG1-Fc & His
Protein Construction
A DNA sequence encoding the human GFRa3 (NP_001487.2)(Met 1-Trp 382) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
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Related Product Information for GFRA3 active protein
Background: Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3/GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA/GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor alpha3 (GFRA3/GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons.
References
Widenfalk J, et al. (2000) Neurturin, RET, GFRalpha-1 and GFRalpha-2, but not GFRalpha-3, mRNA are expressed in mice gonads. Cell Tissue Res. 299(3): 409-15.Li J, et al. (2009) Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice. FEBS Lett. 583(15): 2479-85.Yang C, et al. (2006) Distribution of GDNF family receptor alpha3 and RET in rat and human non-neural tissues. J Mol Histol. 37(1-2): 69-77.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
GDNF family receptor alpha-3 preproprotein
NCBI Official Synonym Full Names
GDNF family receptor alpha 3
NCBI Official Symbol
GFRA3
NCBI Official Synonym Symbols
GDNFR3
NCBI Protein Information
GDNF family receptor alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; GPI-linked receptor; glial cell line-derived neurotrophic factor receptor alpha-3
UniProt Protein Name
GDNF family receptor alpha-3
Protein Family
UniProt Gene Name
GFRA3
UniProt Synonym Gene Names
GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3
UniProt Entry Name
GFRA3_HUMAN
NCBI Description
The protein encoded by this gene is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin. [provided by RefSeq, Jul 2008]
Uniprot Description
GFRA3: Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 5q31.1-q31.3
Cellular Component: extrinsic to membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; external side of plasma membrane
Molecular Function: axon guidance receptor activity; receptor binding
Biological Process: axon guidance; nervous system development; sympathetic nervous system development; neuron migration; signal transduction; peripheral nervous system development
Research Articles on GFRA3
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Product Notes
The GFRA3 gfra3 (Catalog #AAA8120109) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Trp38 2. It is sometimes possible for the material contained within the vial of "GFR Alpha-3/GFRA3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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