Human Glucoprotein 130 (gp130) RTU ELISA Kit | gp130 rtu elisa kit

Human Glucoprotein 130 (gp130) ELISA Kit

Cat. Num. AAA459652

• Gene Names: IL6ST; CD130; GP130; CDW130; IL-6RB
• Reactivity: Human
• Synonyms: Glucoprotein 130 (gp130); Human Glucoprotein 130 (gp130) ELISA Kit; CDw130; CD130; IL6ST; GP130-RAPS; IR6RB; IL6R-Beta; IR6-RB; Interleukin 6 Signal Transducer; Oncostatin M Receptor; Interleukin-6 Receptor Subunit Beta; gp130 rtu elisa kit

• Reactivity: Human
• Sequence Length: 857

• Assay Type: Sandwich
• Samples: Serum, Plasma or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.115ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for gp130 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to gp130. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to gp130. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain gp130, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of gp130 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 300244535
• NCBI GeneID: 3572
• NCBI Accession #: NP_001177910.1
• NCBI GenBank Nucleotide #: NM_001190981.1
• UniProt Accession #: P40189; Q5FC04; Q9UQ41; A0N0L4
• Molecular Weight: 96,276 Da
• NCBI Official Full Name: interleukin-6 receptor subunit beta isoform 3
• NCBI Official Synonym Full Names: interleukin 6 signal transducer
• NCBI Official Symbol: IL6ST
• NCBI Official Synonym Symbols: CD130; GP130; CDW130; IL-6RB
• NCBI Protein Information: interleukin-6 receptor subunit beta
• UniProt Protein Name: Interleukin-6 receptor subunit beta
• UniProt Gene Name: IL6ST
• UniProt Synonym Gene Names: IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; gp130

NCBI Description

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]

Uniprot Description

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates Janus kinases (PubMed:2261637). That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3 (PubMed:19915009, PubMed:23294003). Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (). Has a role in embryonic development (). Does not bind IL6 (PubMed:2261637). Essential for survival of motor and sensory neurons and for differentiation of astrocytes (). Required for expression of TRPA1 in nociceptive neurons (). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (). Required for normal trabecular bone mass and cortical bone composition ().