Mouse anti-Human RPN1 Monoclonal Antibody | anti-RPN1 antibody

RPN1 Mouse mAb

Cat. Num. AAA435181

• Gene Names: RPN1; OST1; RBPH1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: RPN1; Monoclonal Antibody; RPN1 Mouse mAb; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; oligosaccharyltransfe; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); OST1; RBPH1; Ribophorin I; Ribophorin-1; RPN-I; anti-RPN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 607

• Applicable Applications for anti-RPN1 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:5000; IP: 10 tests; FC/FACS: 1:100-1:200
• Source Note: This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human

Western Blot (WB)

(All lanes : RPN1 Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 68 KDa Observed band size : 68 KDa Blocking/Dilution buffer : 1x TBST.)

Immunoprecipitation (IP)

(RPN1 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25011 of 10 ug. Western blot was performed from the immunopre-cipitate using M25011 at 1/2000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Blocking and dilution buffer and concentration:5% milk/TBST.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling RPN1 with M25011 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)

Related Product Information for anti-RPN1 antibody

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser, Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome

Product Categories/Family for anti-RPN1 antibody

Cell Marker; Subcellular Marker; Membrane

NCBI and Uniprot Product Information

• NCBI GI #: 4506675
• NCBI GeneID: 6184
• NCBI Accession #: NP_002941.1
• NCBI GenBank Nucleotide #: NM_002950.3
• UniProt Accession #: P04843; Q68DT1; B2R5Z0; D3DNB6
• Molecular Weight: Predicted MW: 68kDa
• NCBI Official Full Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• NCBI Official Synonym Full Names: ribophorin I
• NCBI Official Symbol: RPN1
• NCBI Official Synonym Symbols: OST1; RBPH1
• NCBI Protein Information: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• UniProt Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
• Protein Family: 26S proteasome regulatory
• UniProt Gene Name: RPN1
• UniProt Synonym Gene Names: RPN-I

NCBI Description

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.