Typical Testing Data/Standard Curve (for reference only)
EZViable Calcein AM Cell Viability Assay Kit
EZViable Calcein AM Cell Viability Assay Kit (Fluorometric)
Synonyms
EZViable Calcein AM Cell Viability; EZViable Calcein AM Cell Viability Assay Kit (Fluorometric); EZViable Calcein AM Cell Viability assay kit
Application Notes
Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients
Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals
Assessment of physiological mediators and antibodies that affect cell growth.
Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals
Assessment of physiological mediators and antibodies that affect cell growth.
Samples
Adherent and suspension cells
Proliferating and non-proliferating cells
Proliferating and non-proliferating cells
Detection Method
Fluorescence (Ex/Em = 485/530 nm)
Features & Benefits
Convenient & non-radioactive
High-throughput
Highly-sensitive: detect as low as 50 viable cells in less than 30 min.
Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis
High-throughput
Highly-sensitive: detect as low as 50 viable cells in less than 30 min.
Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for EZViable Calcein AM Cell Viability assay kit
Product Information: EZViable Calcein AM Cell Viability Assay Kit (Fluorometric): Convenient, non-radioactive & high-throughput assay for quantitating cell viability, cell proliferation, chemotaxis, cytotoxicity and apoptosis. Detects as low as 50 viable cells in less than 30 min. 1000 assays.
Background: Quantification of number of viable cells is an indispensible tool in Cell Biology research. MyBioSource's Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
Summary: Detection method- Fluorescence (Ex/Em = 485/530 nm)
Applications- - Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients - Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals - Assessment of physiological mediators and antibodies that affect cell growth.
Background: Quantification of number of viable cells is an indispensible tool in Cell Biology research. MyBioSource's Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
Summary: Detection method- Fluorescence (Ex/Em = 485/530 nm)
Applications- - Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients - Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals - Assessment of physiological mediators and antibodies that affect cell growth.
Product Categories/Family for EZViable Calcein AM Cell Viability assay kit
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Product Notes
The EZViable Calcein AM Cell Viability (Catalog #AAA841455) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals Assessment of physiological mediators and antibodies that affect cell growth. Researchers should empirically determine the suitability of the EZViable Calcein AM Cell Viability for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EZViable Calcein AM Cell Viability, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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