Typical Testing Data/Standard Curve (for reference only)
2-Phosphoglycerate Assay Kit
2-Phosphoglycerate Colorimetric / Fluorometric Assay Kit
Synonyms
2-Phosphoglycerate; 2-Phosphoglycerate Colorimetric / Fluorometric Assay Kit; 2-Phosphoglyceric Acid Colorimetric/Fluorometric Assay Kit; 2PG Colorimetric/Fluorometric Assay Kit; 2-Phosphoglycerate assay kit
Samples
Animal tissues: e.g. liver, muscle and heart, etc.
Cell culture: adherent or suspension cells
Cell culture: adherent or suspension cells
Features & Benefits
Sensitive, fast, and easy-to-use.
This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for 2-Phosphoglycerate assay kit
Product Information: 2-Phosphoglycerate (2PG) Colorimetric/Fluorometric Assay Kit: The only kit on the market to measure 2PG. Measures 2PG levels in tissues & cells. Detection limit: less than 20 pmol. 100 assays.
Background: 2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research. MyBioSource's 2-Phosphoglycerate Assay kit is a sensitive, fast and easy-to-use kit. In this assay, 2PG is converted by Enzyme Mix to PEP, which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Applications: -Measurement of 2-Phosphoglycerate in various tissues/cells -Analysis of carbohydrate metabolism and cell signaling -Cancer research
Background: 2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research. MyBioSource's 2-Phosphoglycerate Assay kit is a sensitive, fast and easy-to-use kit. In this assay, 2PG is converted by Enzyme Mix to PEP, which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Applications: -Measurement of 2-Phosphoglycerate in various tissues/cells -Analysis of carbohydrate metabolism and cell signaling -Cancer research
Product Categories/Family for 2-Phosphoglycerate assay kit
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Product Notes
The 2-Phosphoglycerate (Catalog #AAA841850) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "2-Phosphoglycerate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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