Testing Data
Human Enolase alpha Assay Kit
Human Enolase alpha Inhibitor Screening Kit (Colorimetric)
Reactivity
Human
Synonyms
Enolase alpha; Human Enolase alpha Inhibitor Screening Kit (Colorimetric); Enolase alpha assay kit
Reactivity
Human
Detection Method
Absorbance: OD 570nm
Features & Benefits
Only kit on the market to screen potential inhibitors of human Enolase
Rapid and easy-to-use.
High-throughput adaptable
Screening/characterizing/studying potential inhibitors of Human Enolase alpha.
This assay kit can be used for initial screening of compounds as anti-Enolase
Rapid and easy-to-use.
High-throughput adaptable
Screening/characterizing/studying potential inhibitors of Human Enolase alpha.
This assay kit can be used for initial screening of compounds as anti-Enolase
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Related Product Information for Enolase alpha assay kit
Enolase (EC 4.2.1.11), also called 2-Phospho-D-glycerate hydrolase, is a key enzyme in glycolysis. It converts 2-phosphoglycerate into phosphoenolpyruvate (PEP). It also catalyzes the reverse reaction, PEP to 2-phosphoglycerate under anabolic conditions during gluconeogenesis. In mammals, three genes encode for the Enolase alpha, beta and gamma isoforms. These isoforms combine together to form both homo and heterodimeric complexes resulting in five isozymes, three of which are commonly found in human tissues. Enolase alpha, also called enolase 1, exists in variety of tissues which can undergo glycolysis. Besides its role in glycolysis, increased Enolase alpha activity is associated with tumor invasion and metastasis; therefore, screening for novel and specific inhibitor of enolase alpha inhibitor may be of great interest for tumor treatment. In Human Enolase alpha Inhibitor Screening Kit, Enolase alpha catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed probe generating a colorimetric signal (OD 570 nm). In the presence of Enolase alpha inhibitor, the reaction is impeded. An enolase alpha Inhibitor Control is included to compare the efficacy of the sample inhibitors. The assay is simple, fast, high-throughput adaptable and can be completed in less than 1 hr
Product Categories/Family for Enolase alpha assay kit
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Product Notes
The Human Enolase alpha (Catalog #AAA849717) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849717 Assay Kit recognizes Human Enolase alpha. It is sometimes possible for the material contained within the vial of "Enolase alpha, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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