Testing Data
Eukaryotes Choline Kinase (ChoK) Assay Kit
Choline Kinase (ChoK) Activity Assay Kit (Fluorometric)
Reactivity
Eukaryotes
Synonyms
Choline Kinase (ChoK); Choline Kinase (ChoK) Activity Assay Kit (Fluorometric); Choline Kinase (ChoK) assay kit
Reactivity
Eukaryotes
Samples
Cell/tissue lysates: kidney, liver
Purified Protein
Purified Protein
Detection Method
Fluorescence: Ex/Em 535/587nm
Features & Benefits
Simple procedure; takes ~ less than 40 minutes
Fast and convenient
Measurement of choline kinase activity in different cell lines and tissues
Sensitive Assay, HTS
Fast and convenient
Measurement of choline kinase activity in different cell lines and tissues
Sensitive Assay, HTS
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Choline Kinase (ChoK) assay kit
Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at lambda = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.
Product Categories/Family for Choline Kinase (ChoK) assay kit
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Product Notes
The Eukaryotes Choline Kinase (ChoK) (Catalog #AAA849693) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849693 Assay Kit recognizes Eukaryotes Choline Kinase (ChoK). It is sometimes possible for the material contained within the vial of "Choline Kinase (ChoK), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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