Chromatin Immunoprecipitation (ChIP)
(AbFlex Histone H3 antibody (rAb) tested by ChIP Chromatin immunoprecipitation (ChIP) was performed with 30 ug of chromatin from Farage human lymph-node derived B cells and 4.5 ug AbFlex Histone H3 antibody. ChIP DNA was used in qPCR with the gene-specific primers as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Rabbit anti-Human Histone H3 Antibody | anti-H3 antibody
AbFlex Histone H3, C-terminal antibody (rAb, Rabbit)
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Protein A Chromatography
Synonyms
Histone H3; AbFlex Histone H3; C-terminal antibody (rAb; Rabbit); anti-H3 antibody
Host
Rabbit
Reactivity
Human
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3 antibody
Chromatin Immunoprecipitation (ChIP), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 4-5ug per ChIP
WB: 0.01-0.5ug/ml
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
ChIP: 4-5ug per ChIP
WB: 0.01-0.5ug/ml
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against a peptide at the C-terminus of human Histone H3.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(AbFlex Histone H3 antibody (rAb) tested by ChIP Chromatin immunoprecipitation (ChIP) was performed with 30 ug of chromatin from Farage human lymph-node derived B cells and 4.5 ug AbFlex Histone H3 antibody. ChIP DNA was used in qPCR with the gene-specific primers as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(AbFlex Histone H3 antibody (rAb) tested by ChIP Chromatin immunoprecipitation (ChIP) was performed with 30 ug of chromatin from Farage human lymph-node derived B cells and 4.5 ug AbFlex Histone H3 antibody. ChIP DNA was used in qPCR with the gene-specific primers as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Western Blot (WB)
(AbFlex Histone H3, C-term antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3, C-term antibody at 0.1 ug/ml.)
Western Blot (WB)
(AbFlex Histone H3, C-term antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3, C-term antibody at 0.1 ug/ml.)
Related Product Information for anti-H3 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Histone H3, C-terminal antibody (rAb, Rabbit) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3, C-term was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in CHO cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4).
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3, C-term was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in CHO cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4).
Product Categories/Family for anti-H3 antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Protein Family
Similar Products
Product Notes
The H3 (Catalog #AAA389504) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Histone H3, C-terminal antibody (rAb, Rabbit) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Western Blot (WB). Applications Validated: ChIP: 4-5ug per ChIP WB: 0.01-0.5ug/ml Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. Researchers should empirically determine the suitability of the H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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