Mouse anti-Human Snail Antibody | anti-Snail antibody

AbFlex Snail antibody (rAb)

Cat. Num. AAA389485

• Gene Names: SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A Chromatography
• Synonyms: Snail; AbFlex Snail antibody (rAb); SNA; SNAH; SLUGH2; SNAIL1; dJ710H13.1; anti-Snail antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG2a
• Purity/Purification: Protein A Chromatography
• Form/Format: Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-Snail antibody: Western Blot (WB)
• Application Notes: Applications Validated:; WB: 0.5-2ug/ml; Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
• Antibody Type: Recombinant Antibody
• Immunogen: This antibody was raised against full length recombinant human SNAI1 protein (snail homolog 1 (Drosophila))
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

SDS-Page

(AbFlex Snail antibody (rAb) tested by Western blot. 20 ug of K562 cell nuclear extract was run on SDS-PAGE and probed with AbFlex Snail antibody at 0.5 ug/ml.)

Related Product Information for anti-Snail antibody

Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Snail antibody (rAb) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human samples.

Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Snail antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Snail (snail homolog 1 (Drosophila)) is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.

Product Categories/Family for anti-Snail antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 18765741
• NCBI GeneID: 6615
• NCBI Accession #: NP_005976; AB_2793821
• NCBI GenBank Nucleotide #: NM_005985.3
• UniProt Accession #: O95863; Q9P113; Q9UBP7; Q9UHH7; B2R842
• Molecular Weight: 29,083 Da
• NCBI Official Full Name: zinc finger protein SNAI1
• NCBI Official Synonym Full Names: snail family transcriptional repressor 1
• NCBI Official Symbol: SNAI1
• NCBI Official Synonym Symbols: SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• NCBI Protein Information: zinc finger protein SNAI1
• UniProt Protein Name: Zinc finger protein SNAI1
• UniProt Gene Name: SNAI1
• UniProt Synonym Gene Names: SNAH; Protein sna
• UniProt Entry Name: SNAI1_HUMAN

NCBI Description

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

Snail1: a protein of the C2H2-type zinc-finger family that regulates transcription. Involved in embryonic mesoderm formation. Plays a role in the epithelial to mesenchymal transition (EMT). EMT is characterized by decreased levels of the epithelial markers E-cadherin and alpha- and beta-catenin, and increased cellular migration and expression of the mesenchymal markers fibronectin, vimentin, N-cadherin and smooth muscle alpha-actin.E Snail binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. NF-kappaB binds and regulates the snail promoter and is critical for EMT, and inhibition of NF-kappaB activity lowered snail levels and morphologically reversed the EMT.

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; kinase binding

Biological Process: osteoblast differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell migration; hair follicle morphogenesis; mesoderm formation; positive regulation of transcription, DNA-dependent; epithelial to mesenchymal transition; negative regulation of transcription from RNA polymerase II promoter; palate development; positive regulation of cell migration