SDS-Page
(AbFlex Rad21 antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Rad21 antibody at 1 ug/ml.)
Mouse anti-Human, Mouse Rad21 Antibody | anti-Rad21 antibody
AbFlex Rad21 antibody (rAb)
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot
Purity
Protein A Chromatography
Synonyms
Rad21; AbFlex Rad21 antibody (rAb); HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21; anti-Rad21 antibody
Host
Mouse
Reactivity
Human, Mouse
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Rad21 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications Validated:
IF: 0.25-1ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
IF: 0.25-1ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against recombinant protein corresponding to the N-terminal half of mouse Rad21.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(AbFlex Rad21 antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Rad21 antibody at 1 ug/ml.)
SDS-Page
(AbFlex Rad21 antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Rad21 antibody at 1 ug/ml.)
Immunofluorescence (IF)
(AbFlex Rad21 antibody (rAb) tested by Immunofluorescence. HeLa cells stained with 0.5 ug /mL of AbFlex Rad21 antibody (rAb) (Top Panel) or DAPI (Lower Panel) followed by anti-mouse IgG-488.)
Immunofluorescence (IF)
(AbFlex Rad21 antibody (rAb) tested by Immunofluorescence. HeLa cells stained with 0.5 ug /mL of AbFlex Rad21 antibody (rAb) (Top Panel) or DAPI (Lower Panel) followed by anti-mouse IgG-488.)
Related Product Information for anti-Rad21 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Rad21 antibody (rAb) was raised in a Mouse host. It has been validated for use in Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Rad21 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells Rad21 (HR21, MCD1, NXP1, hHR21) is highly similar to the gene product of S. pombe Rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. Rad21 is a nuclear phosphoprotein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of Rad21 with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Rad21 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells Rad21 (HR21, MCD1, NXP1, hHR21) is highly similar to the gene product of S. pombe Rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. Rad21 is a nuclear phosphoprotein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of Rad21 with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Product Categories/Family for anti-Rad21 antibody
NCBI and Uniprot Product Information
Molecular Weight
100kDa
Protein Family
Similar Products
Product Notes
The Rad21 (Catalog #AAA389481) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Rad21 antibody (rAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rad21 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). Applications Validated: IF: 0.25-1ug/ml Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the Rad21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad21, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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