SDS-Page
(AbFlex CHD3 antibody (rAb) tested by Western blot. 15 ug of Drosophila Schneider Cell nuclear extract was run on SDS-PAGE and probed with AbFlex CHD3 antibody at 0.5 ug/ml.)
Mouse anti-Drosophila CHD3 Antibody | anti-CHD3 antibody
AbFlex CHD3 antibody (rAb)
Reactivity
Drosophila
Applications
Immunoprecipitation, Western Blot
Purity
Protein A Chromatography
Synonyms
CHD3; AbFlex CHD3 antibody (rAb); EC 3.6.1.3; GD14801; anti-CHD3 antibody
Host
Mouse
Reactivity
Drosophila
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-CHD3 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
IP: 4ug per IP
WB: 0.5-2ug/ml
For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
IP: 4ug per IP
WB: 0.5-2ug/ml
For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acid residues 865-885 of Drosophila.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(AbFlex CHD3 antibody (rAb) tested by Western blot. 15 ug of Drosophila Schneider Cell nuclear extract was run on SDS-PAGE and probed with AbFlex CHD3 antibody at 0.5 ug/ml.)
SDS-Page
(AbFlex CHD3 antibody (rAb) tested by Western blot. 15 ug of Drosophila Schneider Cell nuclear extract was run on SDS-PAGE and probed with AbFlex CHD3 antibody at 0.5 ug/ml.)
Western Blot (WB)
(AbFlex CHD3 antibody (rAb) tested antibody tested by immunoprecipitation. Drosophila Schneider cell nuclear extract was immunoprecipitated using 4 ug of AbFlex CHD3 antibody (Lane 1) or an unrelated AbFlex antibody (Lane 2). Protein from IP was probed by Western blot using AbFlex CHD3 antibody (2 ug/ml).)
Western Blot (WB)
(AbFlex CHD3 antibody (rAb) tested antibody tested by immunoprecipitation. Drosophila Schneider cell nuclear extract was immunoprecipitated using 4 ug of AbFlex CHD3 antibody (Lane 1) or an unrelated AbFlex antibody (Lane 2). Protein from IP was probed by Western blot using AbFlex CHD3 antibody (2 ug/ml).)
Related Product Information for anti-CHD3 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex CHD3 antibody (rAb) was raised in a Mouse host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Drosophila samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex CHD3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. CHD3 (chromodomain helicase DNA binding protein 3) is a component of the CHD family of ATP-dependent chromatin remodelers and forms histone deacetylase NuRD complexes which participate in the remodeling of chromatin by deacetylating histones.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex CHD3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. CHD3 (chromodomain helicase DNA binding protein 3) is a component of the CHD family of ATP-dependent chromatin remodelers and forms histone deacetylase NuRD complexes which participate in the remodeling of chromatin by deacetylating histones.
Product Categories/Family for anti-CHD3 antibody
NCBI and Uniprot Product Information
Molecular Weight
103kDa
Protein Family
Similar Products
Product Notes
The CHD3 (Catalog #AAA389494) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex CHD3 antibody (rAb) reacts with Drosophila and may cross-react with other species as described in the data sheet. AAA Biotech's CHD3 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 4ug per IP WB: 0.5-2ug/ml For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the CHD3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHD3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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