Rabbit anti-Human CYP7A1 Polyclonal Antibody | anti-CYP7A1 antibody

CYP7A1, CT (Cholesterol 7-alpha-hydroxylase, Cholesterol 7-alpha-monooxygenase, CYPVII, Cytochrome P450 7A1, CYP7) (MaxLight 490)

Cat. Num. AAA6471367

• Gene Names: CYP7A1; CP7A; CYP7; CYPVII
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CYP7A1; Polyclonal Antibody; CT (Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase; CYPVII; Cytochrome P450 7A1; CYP7) (MaxLight 490); anti-CYP7A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP7A1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
• Sequence Length: 504

• Applicable Applications for anti-CYP7A1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CYP7A1 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CYP7A1 Antibody (C-term) in K562(lane 1), HepG2(lane 2) cell line lysates (35ug/lane). CYP7A1 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CYP7A1 antibody

Antibodies; Enzymes; Cytochrome

References

1. Lenicek, M., J. Lipid Res. 49(12), 2664-2667 (2008). 2. Nelson, D.R., Pharmacogenetics 14(1), 1-18 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 166295200
• NCBI GeneID: 1581
• NCBI Accession #: NP_000771
• NCBI GenBank Nucleotide #: NP_000771.2
• UniProt Accession #: P22680
• Molecular Weight: 57.661
• NCBI Official Full Name: cytochrome P450 7A1
• NCBI Official Synonym Full Names: cytochrome P450 family 7 subfamily A member 1
• NCBI Official Symbol: CYP7A1
• NCBI Official Synonym Symbols: CP7A; CYP7; CYPVII
• NCBI Protein Information: cytochrome P450 7A1
• UniProt Protein Name: Cholesterol 7-alpha-monooxygenase
• Protein Family: Cholesterol 7-alpha-monooxygenase
• UniProt Gene Name: CYP7A1
• UniProt Synonym Gene Names: CYP7
• UniProt Entry Name: CP7A1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]

Uniprot Description

CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - primary bile acid biosynthesis; Endoplasmic reticulum; Oxidoreductase; EC 1.14.13.17

Chromosomal Location of Human Ortholog: 8q11-q12

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: cholesterol 7-alpha-monooxygenase activity; iron ion binding; heme binding

Biological Process: bile acid biosynthetic process; cholesterol homeostasis; bile acid metabolic process; xenobiotic metabolic process; cellular lipid metabolic process; cholesterol catabolic process; sterol metabolic process; transmembrane transport