SDS-Page
(AbFlex KDM1B / LSD2 antibody tested by Western blot. 20 ug of K562 nuclear extract* was run on SDS-PAGE and probed with AbFlex KDM1B / LSD2 antibody at 0.5 ug/ml.)
Mouse anti-Human KDM1B/LSD2 Antibody | anti-KDM1B/LSD2 antibody
AbFlex KDM1B/LSD2 antibody (rAb)
WB: 0.5-2ug/ml
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. For optimal results, we also recommend the addition of 0.05% Tween-20 to all blocking solutions to reduce background.
SDS-Page
(AbFlex KDM1B / LSD2 antibody tested by Western blot. 20 ug of K562 nuclear extract* was run on SDS-PAGE and probed with AbFlex KDM1B / LSD2 antibody at 0.5 ug/ml.)
SDS-Page
(AbFlex KDM1B / LSD2 antibody tested by Western blot. 20 ug of K562 nuclear extract* was run on SDS-PAGE and probed with AbFlex KDM1B / LSD2 antibody at 0.5 ug/ml.)
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex KDM1B/LSD2 was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells. KDM1B/LSD2 (Lysine (K)-Specific Demethylase 1B/Lysine-Specific Histone Demethylase 2) is a histone demethylase that demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Demethylates both mono- and di-methylated Lys4 of histone H3. Has no effect on tri-methylated Lys4, mono-, di- or tri-methylated Lys9, mono-, di- or tri-methylated Lys27, mono-, di- or tri-methylated Lys36 of histone H3, or on mono-, di- or tri-methylated Lys20 of histone H4 (By similarity).
NCBI and Uniprot Product Information
Observed: 68 kDa
NCBI Description
Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).[supplied by OMIM, Oct 2009]
Uniprot Description
AOF1: Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri- methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys- 36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Belongs to the flavin monoamine oxidase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.-.-.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 6p22.3
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; FAD binding; zinc ion binding; oxidoreductase activity
Biological Process: establishment and/or maintenance of chromatin architecture; genetic imprinting; regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development; DNA methylation during gametogenesis
Research Articles on KDM1B/LSD2
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Product Notes
The KDM1B/LSD2 kdm1b (Catalog #AAA389518) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex KDM1B/LSD2 antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KDM1B/LSD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. For optimal results, we also recommend the addition of 0.05% Tween-20 to all blocking solutions to reduce background. Researchers should empirically determine the suitability of the KDM1B/LSD2 kdm1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM1B/LSD2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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