Mouse Histone H4K20me1 Antibody | anti-H4K20me1 antibody

AbFlex Histone H4K20me1 antibody (rAb)

Cat. Num. AAA389501

• Gene Names: HIST4H4; H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
• Reactivity: Human, Wide Range Predicted
• Applications: Western Blot
• Purity: Protein A Chromatography
• Synonyms: Histone H4K20me1; AbFlex Histone H4K20me1 antibody (rAb); anti-H4K20me1 antibody

• Host: Mouse
• Reactivity: Human, Wide Range Predicted
• Isotype: IgG2a
• Purity/Purification: Protein A Chromatography
• Form/Format: Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
• Concentration: 1ug/ul (varies by lot)

• Applicable Applications for anti-H4K20me1 antibody: Western Blot (WB)
• Application Notes: Applications Validated:; WB: 0.5-2ug/ml; Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
• Antibody Type: Recombinant Antibody
• Conjugation: Methylated
• Immunogen: This antibody was raised against a peptide containing monomethyl-lysine 20 of human Histone H4.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

SDS-Page

(AbFlex Histone H4K20me1 antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H4K20me1 antibody at 0.5 ug/ml.)

Testing Data

(AbFlex Histone H4K20me1 antibody specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20me1 antibody was applied at 0.1 ug/ml to MODified Histone Peptide Array . The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.)

Related Product Information for anti-H4K20me1 antibody

Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Histone H4K20me1 antibody (rAb) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K20me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells. Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/ exit points.

Product Categories/Family for anti-H4K20me1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 28173560
• NCBI GeneID: 121504
• NCBI Accession #: NP_778224
• NCBI GenBank Nucleotide #: NM_175054.2
• UniProt Accession #: P62805; P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0; Q0VAS5; Q6B823
• Molecular Weight: 11,367 Da
• NCBI Official Full Name: histone H4
• NCBI Official Synonym Full Names: histone cluster 4, H4
• NCBI Official Symbol: HIST4H4
• NCBI Official Synonym Symbols: H4/p; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; MGC24116
• NCBI Protein Information: histone H4; histone 4, H4; OTTHUMP00000239323
• UniProt Protein Name: Histone H4
• UniProt Gene Name: HIST1H4A
• UniProt Synonym Gene Names: H4/A; H4FA
• UniProt Entry Name: H4_HUMAN

Uniprot Description

Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular location: Nucleus. Chromosome.

Post-translational modification: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ref.19 Ref.20 Ref.21 Ref.23 Ref.24 Ref.28Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ref.28 Ref.36Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ref.27 Ref.30Sumoylated, which is associated with transcriptional repression. Ref.22Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Ref.35

Sequence similarities: Belongs to the histone H4 family.

Sequence caution: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3.