SDS-Page
(AbFlex Histone H3K4me0 antibody (rAb) tested by Western blot. 20 ug of HeLa nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H3K4me0 antibody at 0.1 ug/ml.)
Rabbit anti-Human Histone H3K4me0 Antibody | anti-H3K4me0 antibody
AbFlex Histone H3K4me0 antibody (rAb, rabbit)
Reactivity
Human
Applications
Dot Blot, Western Blot
Purity
Protein A Chromatography
Synonyms
Histone H3K4me0; AbFlex Histone H3K4me0 antibody (rAb; rabbit); anti-H3K4me0 antibody
Host
Rabbit
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3K4me0 antibody
Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.1-1ug/ml
DB: 0.1-1ug/ml
We also offers AbFlex Histone H3K4me0 antibody (rAb) expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype)
WB: 0.1-1ug/ml
DB: 0.1-1ug/ml
We also offers AbFlex Histone H3K4me0 antibody (rAb) expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype)
Antibody Type
Recombinant Antibody
Conjugation
Methylated
Immunogen
This antibody was raised against a peptide containing the N-terminus of human histone H3.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(AbFlex Histone H3K4me0 antibody (rAb) tested by Western blot. 20 ug of HeLa nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H3K4me0 antibody at 0.1 ug/ml.)
SDS-Page
(AbFlex Histone H3K4me0 antibody (rAb) tested by Western blot. 20 ug of HeLa nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H3K4me0 antibody at 0.1 ug/ml.)
Dot Blot (DB)
(AbFlex Histone H3K4me0 antibody specificity is shown by Dot Blot. Recombinant proteins (ng amounts indicated next to each row) were spotted onto PVDF as follows: Lane 1 - Histone H3 (C110A))))), at 1 ug/ml.)
Dot Blot (DB)
(AbFlex Histone H3K4me0 antibody specificity is shown by Dot Blot. Recombinant proteins (ng amounts indicated next to each row) were spotted onto PVDF as follows: Lane 1 - Histone H3 (C110A))))), at 1 ug/ml.)
Related Product Information for anti-H3K4me0 antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Histone H3K4me0 antibody (rAb, rabbit) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K4me0 was expressed as full-length IgG with Rabbit immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes. This antibody recognizes the Histone H3, lysine 4, which is unmethylated.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K4me0 was expressed as full-length IgG with Rabbit immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes. This antibody recognizes the Histone H3, lysine 4, which is unmethylated.
Product Categories/Family for anti-H3K4me0 antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3K4me0 (Catalog #AAA389513) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Histone H3K4me0 antibody (rAb, rabbit) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3K4me0 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Western Blot (WB). Applications Validated: WB: 0.1-1ug/ml DB: 0.1-1ug/ml We also offers AbFlex Histone H3K4me0 antibody (rAb) expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype). Researchers should empirically determine the suitability of the H3K4me0 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3K4me0, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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