Western Blot (WB)
(AbFlex His-Tag antibody (rAb) tested by Western blot. 250 ng of a His-Tagged protein (Lane 1) or DYKDDDDK-tagged protein (Lane 2: Recombinant Cas9) was run under reducing conditions and probed with AbFlex His-Tag antibody (0.5 ug/ml dilution). The AbFlex His-Tag antibody specifically recognizes only the His-tagged protein.)
Mouse His Antibody | anti-His antibody
AbFlex His-Tag antibody (rAb)
Reactivity
Not Species Specific
Applications
Western Blot
Purity
Protein A Chromatography
Synonyms
His; AbFlex His-Tag antibody (rAb); anti-His antibody
Host
Mouse
Reactivity
Not Species Specific
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-His antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.5-2ug/ml
WB: 0.5-2ug/ml
Antibody Type
Recombinant Antibody
Immunogen
The His-Tag is comprised of a series of six or more histidine residues that are used to label the carboxy or amino terminus of a recombinant protein. This antibody is designed to recognize the His-Tag (HHHHHH).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(AbFlex His-Tag antibody (rAb) tested by Western blot. 250 ng of a His-Tagged protein (Lane 1) or DYKDDDDK-tagged protein (Lane 2: Recombinant Cas9) was run under reducing conditions and probed with AbFlex His-Tag antibody (0.5 ug/ml dilution). The AbFlex His-Tag antibody specifically recognizes only the His-tagged protein.)
Western Blot (WB)
(AbFlex His-Tag antibody (rAb) tested by Western blot. 250 ng of a His-Tagged protein (Lane 1) or DYKDDDDK-tagged protein (Lane 2: Recombinant Cas9) was run under reducing conditions and probed with AbFlex His-Tag antibody (0.5 ug/ml dilution). The AbFlex His-Tag antibody specifically recognizes only the His-tagged protein.)
Related Product Information for anti-His antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex His-Tag antibody (rAb) was raised in a Mouse host. It has been validated for use in Western blot; the sequence is not species specific so it should react with a wide range of sample types.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex His-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The 6xHis-Tag that is normally present on the AbFlex antibody was removed to avoid self binding.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex His-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The 6xHis-Tag that is normally present on the AbFlex antibody was removed to avoid self binding.
Product Categories/Family for anti-His antibody
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The His (Catalog #AAA389457) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex His-Tag antibody (rAb) reacts with Not Species Specific and may cross-react with other species as described in the data sheet. AAA Biotech's His can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml. Researchers should empirically determine the suitability of the His for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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