SDS-Page
(AbFlex Nucleolin antibody (rAb) tested by Western Blot. 25 ug of HeLa cell nuclear extract (Lane 1) or 30 ug K562 cell nuclear extract (Lane 2) was run on SDS-PAGE and probed with AbFlex Nucleolin antibody at 0.5 ug/ml.)
Mouse Nucleolin Antibody
AbFlex Nucleolin antibody (rAb)
Reactivity
Canine, Human
Applications
Western Blot
Purity
Protein A Chromatography
Synonyms
Nucleolin; AbFlex Nucleolin antibody (rAb); Protein C23; Nsr1; anti-Nucleolin antibody
Host
Mouse
Reactivity
Canine, Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Nucleolin antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.1-1ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
WB: 0.1-1ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against a salt-extracted nuclear matrix preparation from MDCK (canine kidney) cells.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(AbFlex Nucleolin antibody (rAb) tested by Western Blot. 25 ug of HeLa cell nuclear extract (Lane 1) or 30 ug K562 cell nuclear extract (Lane 2) was run on SDS-PAGE and probed with AbFlex Nucleolin antibody at 0.5 ug/ml.)
SDS-Page
(AbFlex Nucleolin antibody (rAb) tested by Western Blot. 25 ug of HeLa cell nuclear extract (Lane 1) or 30 ug K562 cell nuclear extract (Lane 2) was run on SDS-PAGE and probed with AbFlex Nucleolin antibody at 0.5 ug/ml.)
Related Product Information for anti-Nucleolin antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex Nucleolin antibody (rAb) was raised in a Mouse host. It has been validated for use in Western blot; it has been shown to react with Canine and Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Nucleolin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be overexpressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Nucleolin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Nucleolin (NCL, C23) is an abundant and ubiquitously expressed protein that is a major component of the nucleolus. Nucleolin seems to be overexpressed in highly proliferative cells and is involved in many aspects of gene expression: chromatin remodeling, DNA recombination and replication, RNA transcription by RNA polymerase I and II (RNA pol II), rRNA processing, mRNA stabilization, cytokinesis and apoptosis. Its role as a histone chaperone has recently been identified. It is involved in the synthesis and maturation of ribosomes, and is located mainly in dense fibrillar regions of the nucleolus.
Product Categories/Family for anti-Nucleolin antibody
NCBI and Uniprot Product Information
Molecular Weight
100kDa
Protein Family
Similar Products
Product Notes
The Nucleolin (Catalog #AAA389477) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex Nucleolin antibody (rAb) reacts with Canine, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleolin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 0.1-1ug/ml Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone. Researchers should empirically determine the suitability of the Nucleolin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleolin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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