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Typical Standard Curve/Testing Data

Human LY75 (Lymphocyte antigen 75) ELISA Kit | LY75 elisa kit

Human LY75 (Lymphocyte antigen 75)

Gene Names
LY75; CD205; LY-75; CLEC13B; DEC-205; GP200-MR6
Reactivity
Human
Synonyms
LY75 (Lymphocyte antigen 75); Human LY75 (Lymphocyte antigen 75); C-type lectin domain family 13 member B/DEC-205/gp200-MR6/CD205; LY75 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of LY75. No significant cross-reactivity or interference between LY75 and analogues was observed.
Sequence Length
1722
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.2-2000pg/ml
Sensitivity
< 2pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LY75were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LY75were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
4 degree C for 6 months

Typical Standard Curve/Testing Data

Typical Standard Curve/Testing Data
Related Product Information for LY75 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LY75antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LY75 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LY75 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LY75 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,183 Da
NCBI Official Full Name
lymphocyte antigen 75
NCBI Official Synonym Full Names
lymphocyte antigen 75
NCBI Official Symbol
LY75
NCBI Official Synonym Symbols
CD205; LY-75; CLEC13B; DEC-205; GP200-MR6
NCBI Protein Information
lymphocyte antigen 75
UniProt Protein Name
Lymphocyte antigen 75
Protein Family
UniProt Gene Name
LY75
UniProt Synonym Gene Names
CD205; CLEC13B; Ly-75
UniProt Entry Name
LY75_HUMAN

Uniprot Description

LY75: Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen- processing compartment. Causes reduced proliferation of B-lymphocytes. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q24

Cellular Component: integral to plasma membrane

Molecular Function: carbohydrate binding; protein binding; receptor activity

Biological Process: endocytosis; immune response; inflammatory response

Research Articles on LY75

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Product Notes

The Human LY75 ly75 (Catalog #AAA766074) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA766074 ELISA Kit recognizes Human LY75. It is sometimes possible for the material contained within the vial of "LY75 (Lymphocyte antigen 75), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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