SDS-Page
(AbFlex JMJD2B / KDM4B antibody (rAb) tested by Western blot. 20 ug of K562 nuclear extract was run on SDS-PAGE and probed with AbFlex JMJD2B / KDM4B antibody at 2 ug/ml.)
Mouse anti-Human JMJD2B/KDM4B Antibody | anti-JMJD2B/KDM4B antibody
AbFlex JMJD2B/KDM4B antibody (rAb)
Gene Names
KDM4B; JMJD2B; TDRD14B
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A Chromatography
Synonyms
JMJD2B/KDM4B; AbFlex JMJD2B/KDM4B antibody (rAb); TDRD14B; anti-JMJD2B/KDM4B antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-JMJD2B/KDM4B antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
IP: 4ug per IP
WB: 0.05-2ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
IP: 4ug per IP
WB: 0.05-2ug/ml
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against full-length recombinant human lysine-specific demethylase 4B (KDM4B) protein.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
SDS-Page
(AbFlex JMJD2B / KDM4B antibody (rAb) tested by Western blot. 20 ug of K562 nuclear extract was run on SDS-PAGE and probed with AbFlex JMJD2B / KDM4B antibody at 2 ug/ml.)
SDS-Page
(AbFlex JMJD2B / KDM4B antibody (rAb) tested by Western blot. 20 ug of K562 nuclear extract was run on SDS-PAGE and probed with AbFlex JMJD2B / KDM4B antibody at 2 ug/ml.)
Western Blot (WB)
(AbFlex JMJD2B / KDM4B antibody (rAb) tested antibody tested by immunoprecipitation. 4 ug of AbFlex JMJD2B / KDM4B antibody (Lane 1) or an unrelated mouse IgG (Lane 2) was used to immunoprecipitate JMJD2B / KDM4B from 500 ug of K562 nuclear extract. The immunoprecipitated protein was detected by Western blotting using a JMJD2B / KDM4B antibody at a dilution of 1:500.)
Western Blot (WB)
(AbFlex JMJD2B / KDM4B antibody (rAb) tested antibody tested by immunoprecipitation. 4 ug of AbFlex JMJD2B / KDM4B antibody (Lane 1) or an unrelated mouse IgG (Lane 2) was used to immunoprecipitate JMJD2B / KDM4B from 500 ug of K562 nuclear extract. The immunoprecipitated protein was detected by Western blotting using a JMJD2B / KDM4B antibody at a dilution of 1:500.)
Related Product Information for anti-JMJD2B/KDM4B antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex JMJD2B/KDM4B antibody (rAb) was raised in a Mouse host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2B/KDM4B antibody was expressed in 293 cells and contains mouse immunoglobulin heavy and light chains. JMJD2B/KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate histone H3 trimethylated at lysine 9. JMJD2B/KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERa-mediated transcriptional activation.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2B/KDM4B antibody was expressed in 293 cells and contains mouse immunoglobulin heavy and light chains. JMJD2B/KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate histone H3 trimethylated at lysine 9. JMJD2B/KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERa-mediated transcriptional activation.
Product Categories/Family for anti-JMJD2B/KDM4B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
122kDa
NCBI Official Full Name
lysine-specific demethylase 4B
NCBI Official Synonym Full Names
lysine demethylase 4B
NCBI Official Symbol
KDM4B
NCBI Official Synonym Symbols
JMJD2B; TDRD14B
NCBI Protein Information
lysine-specific demethylase 4B
UniProt Protein Name
Lysine-specific demethylase 4B
UniProt Gene Name
KDM4B
UniProt Synonym Gene Names
JHDM3B; JMJD2B; KIAA0876
UniProt Entry Name
KDM4B_HUMAN
Uniprot Description
JMJD2B: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. Belongs to the JHDM3 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Demethylase; EC 1.14.11.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; focal adhesion; nucleus; cell junction
Molecular Function: dioxygenase activity; zinc ion binding
Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin modification
Research Articles on JMJD2B/KDM4B
Similar Products
Product Notes
The JMJD2B/KDM4B kdm4b (Catalog #AAA389507) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex JMJD2B/KDM4B antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD2B/KDM4B can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 4ug per IP WB: 0.05-2ug/ml Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. Researchers should empirically determine the suitability of the JMJD2B/KDM4B kdm4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD2B/KDM4B, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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