SDS-Page
(Recombinant YTHDC1 (325-502) SDS PAGE gel 13% SDS-PAGE Coomassie staining MW: 24.9 kDa Purity: >90% )
YTHDC1 recombinant protein
Recombinant YTHDC1 (325-502) protein
Gene Names
YTHDC1; YT521; YT521-B
Synonyms
YTHDC1; Recombinant YTHDC1 (325-502) protein; YTH Domain Containing 1; Splicing Factor YT521; YTHDC1 recombinant protein
Host
E Coli
Form/Format
Recombinant YTHDC1 (325-502) protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant YTHDC1 (325-502) protein is suitable for use in the study of binding assay, inhibitor screening, and selectivity profiling Binding Assay Conditions: 3uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC1 (325-502) protein in 10ul reaction system containing 50mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant YTHDC1 (325-502) SDS PAGE gel 13% SDS-PAGE Coomassie staining MW: 24.9 kDa Purity: >90% )
SDS-Page
(Recombinant YTHDC1 (325-502) SDS PAGE gel 13% SDS-PAGE Coomassie staining MW: 24.9 kDa Purity: >90% )
Testing Data
(HTRF for YTHDC1 (325-502) activity Oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC1 (325-502) protein in a reaction system for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF for YTHDC1 (325-502) activity Oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC1 (325-502) protein in a reaction system for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF for YTHDC1 (325-502) activity Oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC1 (325-502) protein in a reaction system for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF for YTHDC1 (325-502) activity Oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC1 (325-502) protein in a reaction system for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Related Product Information for YTHDC1 recombinant protein
Short Description: Recombinant YTHDC1 (325-502) protein corresponding to amino acids 325-502 that contains the YTH domain sequence of human YTHDC1 (accession number NP_001317627.1) was expressed in E Coli with an N-terminal 6xHis-tag and a C-terminal DYKDDDDK tag. The molecular weight of the protein is 24.9 kDa. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Background: YTHDC1 (YTH Domain Containing 1), is a regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDC1 acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. It specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. YTHDC1 may also regulate alternative splice site selection.
Background: YTHDC1 (YTH Domain Containing 1), is a regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDC1 acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. It specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. YTHDC1 may also regulate alternative splice site selection.
Product Categories/Family for YTHDC1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,692 Da
NCBI Official Full Name
YTH domain-containing protein 1 isoform 3
NCBI Official Synonym Full Names
YTH domain containing 1
NCBI Official Symbol
YTHDC1
NCBI Official Synonym Symbols
YT521; YT521-B
NCBI Protein Information
YTH domain-containing protein 1
UniProt Protein Name
YTH domain-containing protein 1
Protein Family
UniProt Gene Name
YTHDC1
UniProt Synonym Gene Names
KIAA1966; YT521; YT521-B
UniProt Entry Name
YTDC1_HUMAN
Research Articles on YTHDC1
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Product Notes
The YTHDC1 ythdc1 (Catalog #AAA389157) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "YTHDC1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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