Western Blot (WB)
(Figure 1. Western blot analysis of YTHDC1 using anti-YTHDC1 antibody (MBS1753726).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human U937 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human HEPG2 whole cell lysatesLane 5: human HELA whole cell lysatesLane 6: human Jurkat whole cell lysatesLane 7: rat testis tissue lysatesLane 8: rat C6 whole cell lysatesLane 9: mouse testis tissue lysatesLane 10: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-YTHDC1 antigen affinity purified polyclonal antibody (Catalog # MBS1753726) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for YTHDC1 at approximately 85KD. The expected band size for YTHDC1 is at 85KD. )
Rabbit YTHDC1 Polyclonal Antibody | anti-YTHDC1 antibody
Anti-YTHDC1 Antibody
Gene Names
YTHDC1; YT521; YT521-B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
YTHDC1; Polyclonal Antibody; Anti-YTHDC1 Antibody; Ythdc1; Kiaa1966; YTH domain-containing protein 1; YTH domain containing 1; anti-YTHDC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for YTHDC1 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Applicable Applications for anti-YTHDC1 antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml|Human, Mouse, Rat|
Direct ELISA: 0.1-0.5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Immunogen
E Coli-derived human YTHDC1 recombinant protein (Position: S326-K651).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of YTHDC1 using anti-YTHDC1 antibody (MBS1753726).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human U937 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human HEPG2 whole cell lysatesLane 5: human HELA whole cell lysatesLane 6: human Jurkat whole cell lysatesLane 7: rat testis tissue lysatesLane 8: rat C6 whole cell lysatesLane 9: mouse testis tissue lysatesLane 10: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-YTHDC1 antigen affinity purified polyclonal antibody (Catalog # MBS1753726) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for YTHDC1 at approximately 85KD. The expected band size for YTHDC1 is at 85KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of YTHDC1 using anti-YTHDC1 antibody (MBS1753726).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human U937 whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human HEPG2 whole cell lysatesLane 5: human HELA whole cell lysatesLane 6: human Jurkat whole cell lysatesLane 7: rat testis tissue lysatesLane 8: rat C6 whole cell lysatesLane 9: mouse testis tissue lysatesLane 10: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-YTHDC1 antigen affinity purified polyclonal antibody (Catalog # MBS1753726) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for YTHDC1 at approximately 85KD. The expected band size for YTHDC1 is at 85KD. )
Related Product Information for anti-YTHDC1 antibody
YTH domain-containing protein 1 is a protein that in humans is encoded by the YTHDC1 gene. YTHDC1 is a nuclear protein involved in splice site selection that localises to YT bodies; dynamic subnuclear compartments, which first appear at the beginning of S-phase in the cell cycle and disperse during mitosis.
References
1. Hartmann, A. M., Nayler, O., Schwaiger, F. W., Obermeier, A., Stamm, S. The interaction and colocalization of Sam68 with the splicing-associated factor YT521-B in nuclear dots is regulated by the Src family kinase p59(fyn). Molec. Biol. Cell 10: 3909-3926, 1999.
2. Hartz, P. A. Personal Communication. Baltimore, Md. 01/03/2017.
3. Haussmann, I. U., Bodi, Z., Sanchez-Moran, E., Mongan, N. P., Archer, N., Fray, R. G., Soller, M. m(6)A potentiates Sxl alternative pre-mRNA splicing for robust Drosophila sex determination. Nature 540: 301-304, 2016.
2. Hartz, P. A. Personal Communication. Baltimore, Md. 01/03/2017.
3. Haussmann, I. U., Bodi, Z., Sanchez-Moran, E., Mongan, N. P., Archer, N., Fray, R. G., Soller, M. m(6)A potentiates Sxl alternative pre-mRNA splicing for robust Drosophila sex determination. Nature 540: 301-304, 2016.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,692 Da
NCBI Official Full Name
YTH domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
YTH domain containing 1
NCBI Official Symbol
YTHDC1
NCBI Official Synonym Symbols
YT521; YT521-B
NCBI Protein Information
YTH domain-containing protein 1; splicing factor YT521-B; putative splicing factor YT521
UniProt Protein Name
YTH domain-containing protein 1
Protein Family
UniProt Gene Name
YTHDC1
UniProt Synonym Gene Names
KIAA1966; YT521; YT521-B
UniProt Entry Name
YTDC1_HUMAN
Research Articles on YTHDC1
Similar Products
Product Notes
The YTHDC1 ythdc1 (Catalog #AAA1753726) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-YTHDC1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's YTHDC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the YTHDC1 ythdc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YTHDC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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