SDS-Page
Cyclophilin B Active Protein | PPIB active protein
Cyclophilin B, 26- 216aa, Human, E Coli (Bioactivity Validated)
Gene Names
PPIB; OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Cyclophilin B; 26- 216aa; Human; E Coli (Bioactivity Validated); PPIB; Peptidylprolyl isomerase B; CYPB; SCYLP; CYP-S1: Cyclophilin-B; AA553318; AI844835; Cphn 2; Cphn2; CyP 20b; AA408962; CYP S1; MGC14109; MGC2224; peptidyl prolyl cis trans isomerase B; Peptidyl prolyl cis trans isomerase B precursor; PPIase; Rotamase; S cyclophilin.; PPIB active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
In 20mM Tris 8.0, .20mM NaCl, 0.5mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE
Sequence Length
216
Applicable Applications for PPIB active protein
SDS-PAGE
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity is > 1,500 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PPIB active protein
Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E Coli and purified by using conventional chromatography techniques
Product Categories/Family for PPIB active protein
References
Watashi K, et al. (2005) Mol Cell. 19 (1):111-22. ; Obata Y, et al. (2005) J Biol Chem. 280 (18):18355-60; Denys A, et al. (1997) Immunology. 91 (4):609-17.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21.2kDa (192aa), confirmed by MALDI-TOF.
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase B
NCBI Official Synonym Full Names
peptidylprolyl isomerase B
NCBI Official Symbol
PPIB
NCBI Official Synonym Symbols
OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase B
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase B
Protein Family
UniProt Gene Name
PPIB
UniProt Synonym Gene Names
CYPB; PPIase B; SCYLP
NCBI Description
The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009]
Uniprot Description
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Research Articles on PPIB
Similar Products
Product Notes
The PPIB ppib (Catalog #AAA203068) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cyclophilin B can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PPIB ppib for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE. It is sometimes possible for the material contained within the vial of "Cyclophilin B, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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