Rabbit anti-Human, Mouse YTHDC1 Polyclonal Antibody | anti-YTHDC1 antibody

YTHDC1 Antibody

Cat. Num. AAA7050984

• Gene Names: YTHDC1; YT521; YT521-B
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G purified
• Synonyms: YTHDC1; Polyclonal Antibody; YTHDC1 Antibody; YTH domain-containing protein 1Curated; Splicing factor YT5211; anti-YTHDC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-YTHDC1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1: 1000-1: 5000
• Immunogen: Recombinant human YTH domain-containing protein 1 protein (89-153AA)
• Species: Homo sapiens (Human)
• Research Area: Epigenetics and Nuclear Signaling
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-160929 / sc-160931
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse lung tissue,U251 whole cell lysateAll lanes: YTHDC1 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 85,83 kDaObserved band size: 85 kDa)

Related Product Information for anti-YTHDC1 antibody

Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed: 26318451, PubMed: 26876937, PubMed: 25242552). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed: 26318451, PubMed: 25242552). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed: 26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed: 26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed: 26876937). May also regulate alternative splice site selection (PubMed: 20167602).

NCBI and Uniprot Product Information

• NCBI GI #: 72534750
• NCBI GeneID: 91746
• NCBI Accession #: NP_001026902.1
• NCBI GenBank Nucleotide #: NM_001031732.3
• UniProt Accession #: Q96MU7; Q4W5Q3; Q7Z622; Q8TF35
• NCBI Official Full Name: YTH domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: YTH domain containing 1
• NCBI Official Symbol: YTHDC1
• NCBI Official Synonym Symbols: YT521; YT521-B
• NCBI Protein Information: YTH domain-containing protein 1
• UniProt Protein Name: YTH domain-containing protein 1
• Protein Family: YTH domain-containing protein
• UniProt Gene Name: YTHDC1
• UniProt Synonym Gene Names: YT521-B
• UniProt Entry Name: YTDC1_HUMAN

Uniprot Description

Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed:26318451, PubMed:26876937, PubMed:25242552). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:26318451, PubMed:25242552). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937). May also regulate alternative splice site selection (PubMed:20167602).