SDS-Page
(Recombinant YTHDF3 (407-560) SDS PAGE gel 12.5% SDS-PAGE gel with Coomassie Bluestaining MW: 22.6 kDa Purity: >80% )
YTHDF3 recombinant protein
Recombinant YTHDF3 (407-560) protein
Synonyms
YTHDF3; Recombinant YTHDF3 (407-560) protein; YTH N6-Methyladenosine RNA Binding Protein 3; YTHDF3 recombinant protein
Host
E Coli
Form/Format
Recombinant YTHDF3 (407-560) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 20% glycerol, 2mM DTT, 0.04% Triton X-100.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant YTHDF3 (407-560) protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant YTHDF3 (407-560) SDS PAGE gel 12.5% SDS-PAGE gel with Coomassie Bluestaining MW: 22.6 kDa Purity: >80% )
SDS-Page
(Recombinant YTHDF3 (407-560) SDS PAGE gel 12.5% SDS-PAGE gel with Coomassie Bluestaining MW: 22.6 kDa Purity: >80% )
Testing Data
(HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF for YTHDF3 (407-560) activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF3 (407-560) protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Related Product Information for YTHDF3 recombinant protein
Short Description: Recombinant human YTHDF3 (407-560) protein corresponding to amino acids 407-560 that contains the YTH domain sequence of human YTHDF3 (accession number NP_689971.4) was expressed in E Coli with an N-terminal 6xHis-tag and a C-terminal DYKDDDDK tag. The molecular weight of the protein is 22.6 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: YTHDF3 (YTH N6-Methyladenosine RNA Binding Protein 3) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs and promotes RNA translation efficiency, sharing m6A-containing mRNAs targets with YTHDF1 and YTHDF2, and regulates different processes depending on the context. It facilitates the translation of targeted mRNAs in cooperation with YTHDF1 by binding to m6A-containing mRNAs and interacting with 40S and 60S ribosome subunits. It can also act as a regulator of mRNA stability in cooperation with YTHDF2 by binding to m6A-containing mRNA and promoting their degradation. YTHDF3 recognizes and binds m6A-containing circular RNAs (circRNAs) and promotes their translation. circRNAs are generated through back-splicing of pre-mRNAs, a non-canonical splicing process promoted by dsRNA structures across circularizing exons. N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messenger RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).
Background: YTHDF3 (YTH N6-Methyladenosine RNA Binding Protein 3) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs and promotes RNA translation efficiency, sharing m6A-containing mRNAs targets with YTHDF1 and YTHDF2, and regulates different processes depending on the context. It facilitates the translation of targeted mRNAs in cooperation with YTHDF1 by binding to m6A-containing mRNAs and interacting with 40S and 60S ribosome subunits. It can also act as a regulator of mRNA stability in cooperation with YTHDF2 by binding to m6A-containing mRNA and promoting their degradation. YTHDF3 recognizes and binds m6A-containing circular RNAs (circRNAs) and promotes their translation. circRNAs are generated through back-splicing of pre-mRNAs, a non-canonical splicing process promoted by dsRNA structures across circularizing exons. N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messenger RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).
Product Categories/Family for YTHDF3 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of the protein is 22.6kDa
Protein Family
Similar Products
Product Notes
The YTHDF3 (Catalog #AAA389161) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "YTHDF3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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