SDS-Page
(Recombinant IGF2BP2 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 67.4 kDa Purity: >88%)
IGF2BP2 recombinant protein
Recombinant IGF2BP2 protein
Gene Names
IGF2BP2; IMP2; IMP-2; VICKZ2
Synonyms
IGF2BP2; Recombinant IGF2BP2 protein; VICKZ2; IMP2; Insulin-like growth factor 2 mRNA-binding protein 2; IGF2BP2 recombinant protein
Host
Baculovirus
Form/Format
Recombinant IGF2BP2 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant IGF2BP2 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 67.4 kDa Purity: >88%)
SDS-Page
(Recombinant IGF2BP2 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 67.4 kDa Purity: >88%)
Testing Data
(HTRF for IGF2BP2 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. .)
Testing Data
(HTRF for IGF2BP2 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. .)
Testing Data
(HTRF for IGF2BP2 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(HTRF for IGF2BP2 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Related Product Information for IGF2BP2 recombinant protein
Short Description: Full-length human IGF2BP2 protein (accession number NP_006539.3) was expressed in a baculovirus system with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 67.4 kDa.
Background: IGF2BP2 (Insulin Like Growth Factor 2 MRNA Binding Protein 2), also called as VICKZ2 or IMP2, is a member of the insulin-like growth factor 2 mRNA-binding protein family. The mammalian IGF2 mRNA binding proteins (IGF2BPs) family encompasses three RNA-binding proteins (RBPs) controlling the cytoplasmic fate of mRNAs in development, somatic cells and human diseases. IGF2BPs are RNA-binding factors that recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Recent studies revealed that the association of IGF2BPs with target mRNAs, e.g. the MYC mRNA, is enhanced by the N6-methyladenosine (m6A) modification of target transcripts suggesting IGF2BPs as novel m6A-readers. N6- methyladenosine (m6A) is the most prevalent modification in eukaryotic RNAs. The biological importance of m6A relies on m6A readers, which control mRNA fate and function. Besides, it is suggested that only IGF2BP2 (but not other members of the IGF2BP family or the YTH family) directly bounds to the specific m6A sites in SOX2 CDS regions and controlled the SOX2 mRNA half-life via an m6A-dependent manner. In fact, before its identification as an m6A reader, IGF2BP2 had already suggested to be associated with tumor progression through preserving the stemness phenotype in glioblastoma and hepatocellular carcinoma.
Background: IGF2BP2 (Insulin Like Growth Factor 2 MRNA Binding Protein 2), also called as VICKZ2 or IMP2, is a member of the insulin-like growth factor 2 mRNA-binding protein family. The mammalian IGF2 mRNA binding proteins (IGF2BPs) family encompasses three RNA-binding proteins (RBPs) controlling the cytoplasmic fate of mRNAs in development, somatic cells and human diseases. IGF2BPs are RNA-binding factors that recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Recent studies revealed that the association of IGF2BPs with target mRNAs, e.g. the MYC mRNA, is enhanced by the N6-methyladenosine (m6A) modification of target transcripts suggesting IGF2BPs as novel m6A-readers. N6- methyladenosine (m6A) is the most prevalent modification in eukaryotic RNAs. The biological importance of m6A relies on m6A readers, which control mRNA fate and function. Besides, it is suggested that only IGF2BP2 (but not other members of the IGF2BP family or the YTH family) directly bounds to the specific m6A sites in SOX2 CDS regions and controlled the SOX2 mRNA half-life via an m6A-dependent manner. In fact, before its identification as an m6A reader, IGF2BP2 had already suggested to be associated with tumor progression through preserving the stemness phenotype in glioblastoma and hepatocellular carcinoma.
Product Categories/Family for IGF2BP2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,578 Da
NCBI Official Full Name
insulin-like growth factor 2 mRNA-binding protein 2 isoform a
NCBI Official Synonym Full Names
insulin-like growth factor 2 mRNA binding protein 2
NCBI Official Symbol
IGF2BP2
NCBI Official Synonym Symbols
IMP2; IMP-2; VICKZ2
NCBI Protein Information
insulin-like growth factor 2 mRNA-binding protein 2; VICKZ family member 2; IGF2 mRNA-binding protein 2; IGF-II mRNA-binding protein 2
Protein Family
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Product Notes
The IGF2BP2 (Catalog #AAA389335) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IGF2BP2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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