Western Blot (WB)
(Western Blot analysis of SFRS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:Lane 1: Mouse testis tissue lysateLane 2: Rat brain tissue lysateLane 3: A431 cell lysateLane 4: SH-SY-5Y cell lysateLane 5: SiHa cell lysateLane 6: 293 cell lysateLane 7: Hela cell lysateLane 8: HepG2 cell lysateLane 9: A549 cell lysate)
Rabbit SFRS3 Polyclonal Antibody | anti-SFRS3 antibody
SFRS3 Polyclonal Antibody
ICC: 1:50-1:200
IHC: 1:100-1:500
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of SFRS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:Lane 1: Mouse testis tissue lysateLane 2: Rat brain tissue lysateLane 3: A431 cell lysateLane 4: SH-SY-5Y cell lysateLane 5: SiHa cell lysateLane 6: 293 cell lysateLane 7: Hela cell lysateLane 8: HepG2 cell lysateLane 9: A549 cell lysate)
Western Blot (WB)
(Western Blot analysis of SFRS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control:Lane 1: Mouse testis tissue lysateLane 2: Rat brain tissue lysateLane 3: A431 cell lysateLane 4: SH-SY-5Y cell lysateLane 5: SiHa cell lysateLane 6: 293 cell lysateLane 7: Hela cell lysateLane 8: HepG2 cell lysateLane 9: A549 cell lysate)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SFRS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8. 0-8. 4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-47 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SFRS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8. 0-8. 4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-47 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010]
Uniprot Description
SFRS3: May be involved in RNA processing in relation with cellular proliferation and/or maturation. Belongs to the splicing factor SR family.
Protein type: Spliceosome; RNA-binding; RNA processing; RNA splicing
Chromosomal Location of Human Ortholog: 6p21
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein binding; RNA binding; nucleotide binding
Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription
Research Articles on SFRS3
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Product Notes
The SFRS3 srsf3 (Catalog #AAA3013438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SFRS3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SFRS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:500-1:2,000 ICC: 1:50-1:200 IHC: 1:100-1:500. Researchers should empirically determine the suitability of the SFRS3 srsf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFRS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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