YTHDF3 recombinant protein

Recombinant YTHDF3 protein

Cat. Num. AAA389254

• Synonyms: YTHDF3; Recombinant YTHDF3 protein; YTH N6-Methyladenosine RNA Binding Protein 3; YTHDF3 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant YTHDF3 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: Recombinant YTHDF3 protein is suitable for use in the study of binding assay, inhibitor screening,and selectivity profiling. HTRF assay was used for analysis of YTHDF3 protein activity 3uM biotinylated m6A ssDNA oligos (GTTGG/m6A/CTT) were incubated with different concentrations of YTHDF3 protein in a 10ul system containing 50mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant YTHDF3 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 65.8 kDa Purity: >90%)

Testing Data

(HTRF assay for YTHDF3 protein activity 3 uM biotinylated m6A ssDNA oligos (GTTGG/m6A/CTT) were incubated with different concentrations of YTHDF3 protein in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Testing Data

(HTRF assay for YTHDF3 protein activity 3 uM biotinylated m6A ssDNA oligos (GTTGG/m6A/CTT) were incubated with different concentrations of YTHDF3 protein in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Related Product Information for YTHDF3 recombinant protein

Short Description: Recombinant YTHDF3 protein was expressed in a baculovirus expression system as the full-length human protein (accession number NP_001264742.1) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 65.8 kDa. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Background: YTHDF3 (YTH N6-Methyladenosine RNA Binding Protein 3) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs and promotes RNA translation efficiency, sharing m6A-containing mRNAs targets with YTHDF1 and YTHDF2, and regulates different processes depending on the context. It facilitates the translation of targeted mRNAs in cooperation with YTHDF1 by binding to m6A-containing mRNAs and interacting with 40S and 60S ribosome subunits. It can also act as a regulator of mRNA stability in cooperation with YTHDF2 by binding to m6A-containing mRNA and promoting their degradation. YTHDF3 recognizes and binds m6A-containing circular RNAs (circRNAs) and promotes their translation. circRNAs are generated through back-splicing of pre-mRNAs, a non-canonical splicing process promoted by dsRNA structures across circularizing exons. N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messenger RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).

Product Categories/Family for YTHDF3 recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 481026555
• NCBI GeneID: 253943
• NCBI Accession #: NP_001264742.1
• NCBI GenBank Nucleotide #: NM_001277813.1
• UniProt Accession #: Q7Z739; Q63Z37; Q659A3; B3KXL4
• Molecular Weight: 63,861 Da
• NCBI Official Full Name: YTH domain-containing family protein 3 isoform b
• NCBI Official Synonym Full Names: YTH N(6)-methyladenosine RNA binding protein 3
• NCBI Official Symbol: YTHDF3
• NCBI Protein Information: YTH domain-containing family protein 3
• UniProt Protein Name: YTH domain-containing family protein 3
• Protein Family: YTH domain-containing family protein
• UniProt Gene Name: YTHDF3
• UniProt Entry Name: YTHD3_HUMAN

NCBI Description

This gene encodes a member of the YTH (YT521-B homology) domain protein family. The YTH domain is common in eukaryotes, is often found in the middle of the protein sequence, and may function in binding to RNA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

YTHDF3: Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 8q12.3

Molecular Function: protein binding