ELISA (EIA)
(Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, 9153. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL)
Rabbit COVID 19 3CL-PRO (NSP5) Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody
Reactivity
Virus
Applications
ELISA, Western Blot
Purity
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is affinity chromatography purified via peptide column
Synonyms
COVID 19 3CL-PRO (NSP5) Coronavirus; Polyclonal Antibody; SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; 3CL-PRO (NSP5); anti-COVID-19 antibody
Host
Rabbit
Reactivity
Virus
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is affinity chromatography purified via peptide column
Form/Format
Liquid
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide.
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 0.5ug/mL
Antibody validated: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1ug/mL in ELISA.
Antibody validated: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1ug/mL in ELISA.
Immunogen
Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody (9153) was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.
The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.
The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.
Homology
Predicted reactivity based on immunogen sequence: SARS-CoV 3CL-PRO (NSP5) protein: (100%)
Conjugation
Unconjugated
Preparation and Storage
SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ELISA (EIA)
(Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, 9153. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL)
ELISA (EIA)
(Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, 9153. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL)
Western Blot (WB)
(Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) recombinant protein (10-400).Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL)
Western Blot (WB)
(Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) recombinant protein (10-400).Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL)
Related Product Information for anti-COVID-19 antibody
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. 3C- like proteinase (NSP5) cleaves the C-terminus of replicase polyprotein at 11 sites (3,4,5). It recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. It also able to bind an ADP-ribose-1''-phosphate (ADRP) (5).
Product Categories/Family for anti-COVID-19 antibody
References
Gorbalenya. bioRxiv: 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Zhang et al. Science. 2020; 0:0-0(2020)
Jin et al. Nature, 0:0-0(2020)
Dai et al. Science 0:0-0(2020)
Hui et al. Int J Infect Dis. 2020;91:264-266.
Zhang et al. Science. 2020; 0:0-0(2020)
Jin et al. Nature, 0:0-0(2020)
Dai et al. Science 0:0-0(2020)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Similar Products
Product Notes
The COVID-19 (Catalog #AAA154659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 3CL-PRO (NSP5) Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 0.5ug/mL Antibody validated: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody can detect 2 ng of free peptide at 1ug/mL in ELISA. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 3CL-PRO (NSP5) Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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