SDS-Page
(Recombinant SETD1A-SET Complex 10% SDS-PAGE with Coomassie blue staining MW of SETD1A-SET: 35.2 kDa WDR5 of 36.6 kDa RBBP5 of 59.2 kDa ASH2L of 68.7 kDa Purity: >95%)
SETD1A-SET recombinant protein
Recombinant SETD1A-SET complex
Synonyms
SETD1A-SET; Recombinant SETD1A-SET complex; Histone-lysine N-methyltransferase SETD1A; KMT2F; SETD1A-SET recombinant protein
Host
Baculovirus
Form/Format
Recombinant SETD1A-SET Complex is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.
Protein Species
Human
Notes
This complex is suitable for use in protein-protein interaction, in vitro transcription assay, binding assays.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SETD1A-SET Complex 10% SDS-PAGE with Coomassie blue staining MW of SETD1A-SET: 35.2 kDa WDR5 of 36.6 kDa RBBP5 of 59.2 kDa ASH2L of 68.7 kDa Purity: >95%)
SDS-Page
(Recombinant SETD1A-SET Complex 10% SDS-PAGE with Coomassie blue staining MW of SETD1A-SET: 35.2 kDa WDR5 of 36.6 kDa RBBP5 of 59.2 kDa ASH2L of 68.7 kDa Purity: >95%)
Testing Data
(HTRF assay for SETD1A-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1A-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)
Testing Data
(HTRF assay for SETD1A-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1A-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)
Testing Data
(HTRF assay for SETD1A-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1A-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)
Testing Data
(HTRF assay for SETD1A-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1A-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)
Related Product Information for SETD1A-SET recombinant protein
Short Description: SETD1A (SET Domain Containing 1A, Histone Lysine Methyltransferase) is a component of a histone methyltransferase (HMT) complex (contain SETD1A-SET ASH2L, RBBP5, WDR5) that produces mono-, di-, and trimethylated histone H3 at Lys4, but not if the neighboring 'Lys-9' residue is already methylated. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily. This complex is suitable for use in protein-protein interaction, in vitro transcription assay, binding assays.
Background: SETD1A (SET Domain Containing 1A, Histone Lysine Methyltransferase) is a component of a histone methyltransferase (HMT) complex (contain SETD1A-SET ASH2L, RBBP5, WDR5) that produces mono-, di-, and trimethylated histone H3 at Lys4, but not if the neighboring 'Lys-9' residue is already methylated. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily.
Background: SETD1A (SET Domain Containing 1A, Histone Lysine Methyltransferase) is a component of a histone methyltransferase (HMT) complex (contain SETD1A-SET ASH2L, RBBP5, WDR5) that produces mono-, di-, and trimethylated histone H3 at Lys4, but not if the neighboring 'Lys-9' residue is already methylated. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily.
Product Categories/Family for SETD1A-SET recombinant protein
Similar Products
Product Notes
The SETD1A-SET (Catalog #AAA389385) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SETD1A-SET, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
