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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
5464 results for "Secondary Antibodies Monoclonal" - showing 1-50
Western Blot (WB)
(Western Blot: Lysates were prepared from human liver in reducing or non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to a PVDF membrane. The blot was developed with 2ug/ml of MBS604737 and chemiluminescent detection substrate.)
a1-Acid Glycoprotein, Monoclonal Antibody (Cat# AAA604737)
Full Name
a1-Acid Glycoprotein (Orosomucoid)
Gene Names
Orm1; AGP; OMD; Orm; Agpa1
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Purified by Protein A affinity chromatography from culture supernatant.
Pricing
$800/0.1 mg | $3,590/5x0.1 mg
Western Blot
(SARS-CoV-2(COVID-19) Nucleoprotein Antibody [3863])
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154642)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation with HumanRecombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, 35-710, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ?g/mL Lane 2: 1?g/mL)
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154641)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Western Blot (WB)
(Western Blot shows lysates of human ovarian cancer tissue. PVDF membrane was probed with MBS606917 (2ug/ml) followed by HRP-conjugated anti-mouse IgG secondary antibody. A specific band was detected for ADAMTS1 at ~60-70kD (as indicated). This experiment was conducted under non-reducing conditions and using Blotting Buffer: 25 mM Tris, pH 7.4, 0.15 M NaCl, 0.1% Tween 20; Blocking Solution: 5% nonfat dry milk in Blotting Buffer, Adjust to pH 7.4; Antibody Solution: 2% nonfat dry milk in Blotting Buffer, Adjust to pH 7.4.)
ADAMTS1, Monoclonal Antibody (Cat# AAA606917)
Full Name
ADAMTS1 (A Disintegrin And Metalloproteinase with ThromboSpondin-1 motif, METH-1)
Gene Names
ADAMTS1; C3-C5; METH1; KIAA1346
Reactivity
Human
Applications
EL/EIA, WB, IP
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$800/0.1 mg | $3,590/5x0.1 mg
Western Blot (WB)
(Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, MBS154639, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ug/mL Lane 2: 1ug/mL)
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154639)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF, WB
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Westen Blot (WB)
(Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, 35-712, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 0.5 ?g/mL Lane 2: 1?g/mL)
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154643)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Testing Data
CD 20, Monoclonal Antibody (Cat# AAA684044)
Full Name
Anti - CD 20
Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Reactivity
Human
Applications
IHC
Pricing
$380/7 mL (RTU) | $360/0.1 mL (Concentrate) | $585/0.5 mL (Concentrate) | $890/1 mL (Concentrate) | $470/0.2 mL (Concentrate) | $550/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $3,745/5x1 mL (Concentrate)
Western Blot (WB)
(Figure 1 Western Blot Validation with Human Recombinant ProteinLoading: 50 ng per lane of humannucleocapsid recombinant protein.Antibodies: SARS-CoV-2 nucleocapsid, 35-580, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 1 ug/mL Lane 2: 2ug/mL)
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA154640)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
EIA, IF, WB
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
$625/0.1 mg | $2,815/5x0.1 mg
Western Blot (WB)
(Detection of Human ADAMTS15 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2ug/ml of Human ADAMTS15 Monoclonal Antibody followed by HRP- conjugated Mouse IgG Secondary Antibody. A specific band was detected for mature ADAMTS15 at approximately 75kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
ADAMTS15, Monoclonal Antibody (Cat# AAA6001180)
Full Name
ADAMTS15 (A Disintegrin And Metalloproteinase With Thrombospondin Motifs 15, ADAM-TS 15, ADAM-TS15, ADAMTS-15)
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Affinity Purified
Purified by Protein A/G affinity chromatography.
Purified by Protein A/G affinity chromatography.
Pricing
$735/0.1 mg | $3,290/5x0.1 mg
Immunohistochemistry (IHC)
(Human Kidney, Red Blood Cells (formalin-fixed, paraffin-embedded) stained with GYPA antibody at 1:50 followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
CD235ab, Monoclonal Antibody (Cat# AAA6011605)
Full Name
CD235ab (GYPA, Glycophorin-A, MN Sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GPA/GYPB, Glycophorin-B, PAS-3, SS-active Sialoglycoprotein, Sialoglycoprotein delta, GPB)
Reactivity
Human
Applications
IHC, FC/FACS
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$735/0.05 mg | $3,290/5x0.05 mg
Immunohistchemistry (IHC)
(HRS cells of the classical Hodgkin Lymphoma showing cytoplasmic expression of the EBV LMP-1 protein. Formalin fixed, paraffin embedded human tissue (4 um section) stained with anti - EBVLMP-1 (MBS684137))
EBV/LMP-1, Monoclonal Antibody (Cat# AAA684137)
Full Name
Anti - EBV/LMP-1
Reactivity
Human
Applications
IHC
Pricing
$440/7 mL (RTU) | $405/0.1 mL (Concentrate) | $695/0.5 mL (Concentrate) | $1,075/1 mL (Concentrate) | $555/0.2 mL (Concentrate) | $645/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $4,575/5x1 mL (Concentrate)
Cervical lymph node metastasis of oropharyngeal HPV-associated squamous cell
((A), high grade squamous intraepithelial lesion of the uterine cervix (in contrast with normal endocervical glands))
p16, Monoclonal Antibody (Cat# AAA684198)
Full Name
Anti - p16
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
IHC
Pricing
$375/7 mL (RTU) | $355/0.1 mL (Concentrate) | $580/0.5 mL (Concentrate) | $875/1 mL (Concentrate) | $465/0.2 mL (Concentrate) | $540/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $3,675/5x1 mL (Concentrate)
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA214794)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC, IF
Pricing
$195/0.025 mg | $730/5x0.025 mg
Flow Cytometry (FC/FACS)
( Cell Surface FLOW analysis of PD-L1 in PHA treated human PBMC using 1 ug of PD-L1 antibody (Clone:ABM4E54). Green represents isotype control; red represents anti-PD-L1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
PD-L1, Monoclonal Antibody (Cat# AAA668452)
Full Name
Monoclonal Antibody to PD-L1 (Clone: ABM4E54)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
IHC, FC/FACS, WB
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $165/0.025 mg | $1,405/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA212026)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS, IF
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Western Blot (WB)
(Western Blot Analysis Raji Cell Lysate, Vimentin Mouse Monoclonal Antibody (VM452))
Vimentin, Monoclonal Antibody (Cat# AAA438228)
Full Name
Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody
Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human, Cow, Dog, Cat, Pig, Goat, Chicken.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western Blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TNF-alpha treated, using using MBS633051, NFkB p65, phosphorylated antibody (upper) or N2302-04, NFkB p65 antibody (lower).)
NFkB, p65, Monoclonal Antibody (Cat# AAA633051)
Full Name
NFkB, p65, phosphorylated (Ser536) (NF Kappa B, NF-kB, Nuclear Factor kappa B)
Gene Names
NKIRAS2; KBRAS2; kappaB-Ras2
Reactivity
Hamster, Human, Monkey, Mouse, Porcine, Rat
Applications
WB, IP, FC/FACS, IF
Purity
Supernatant
Supernatant
Supernatant
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808982)
Full Name
Alpha Synuclein Antibody, Clone 4F1: FITC
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Testing Data
(Figure 1 Overexpression Validation of PD-L1 in 293 CellsLoading: 15 μg of lysates per lane.Antibodies: (A, 0.25 μg/mL; B, 0.5 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.)
PDL1, Monoclonal Antibody (Cat# AAA154572)
Full Name
PDL1 Antibody [6H10]
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
EIA, WB, IHC, ICC, IF
Purity
Protein A purified
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Immunohistochemistry - Paraffin (IHC)
(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
FOLH1 / PSMA, Monoclonal Antibody (Cat# AAA249360)
Full Name
Anti-FOLH1 / PSMA Antibody (clone 3H5) IHC-plus
Gene Names
FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
Reactivity
Mouse, Human
Applications
IHC, IF, WB, FC
Purity
Protein A/G purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Western Blot (WB)
(Anti-ITA6 at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 127 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
ITA6, Monoclonal Antibody (Cat# AAA9200194)
Full Name
ITA6 Antibody
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human
Applications
IF, EIA, WB, FC/FACS, IHC-P
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Testing Data
(Published customer image: Optimization of secondary antibody staining concentration for detection of CADO48A. Cells were stained with the primary antibody CADO48A alone followed by (a) 1:10 (b) 1:50 and (c) 1:100 dilutions of a secondary FITC antibody. Optimal detection was seen between 1:50 and 1:100 dilution with CADO48A alone. Secondary FITC concentrations of 1:50 (d) and 1:100 (e) to detect CADO48A on pre-labeled CD11b cells were then evaluated and demonstrated that a 1:50 concentration of FITC was optimal for optimal detection of distinct CADO48A+ cells. Based on these findings, a 1:50 FITC concentration was used in all clinical samples. All cells in these diagrams were gated on P1.From: Sherger et al. BMC Veterinary Research 2012 8:209)
CD11b, Monoclonal Antibody (Cat# AAA215215)
Full Name
MOUSE ANTI DOG CD11b
Gene Names
ITGAM; CD11B
Applications
FC/FACS, IP
Pricing
$575/2 mL | $2,445/5x2 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analyis of SSTR2 in human brain. SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using MBS600002 (3ug/ml) overnight at 4°C. Tissue was stained with the Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).)
Somatostatin Receptor Type 2, Monoclonal Antibody (Cat# AAA600002)
Full Name
Somatostatin Receptor Type 2 (SSTr2)
Gene Names
SSTR2; SSTR2
Reactivity
Human
Applications
IHC, ICC, FC/FACS
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Pricing
$835/0.1 mg | $3,750/5x0.1 mg
Immunohistochemistry (IHC)
(Anti-PECAM-1 / CD31 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)
PECAM-1 / CD31, Monoclonal Antibody (Cat# AAA249056)
Full Name
Mouse Monoclonal [clone 1H6] (IgG1) to Human PECAM-1 / CD31
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human, Dog
Applications
IHC, IF, WB, FC/FACS
Purity
Immunoaffinity purified from tissue culture supernatant
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Immunohistochemistry (IHC)
(Anti-LAMP2 / CD107b antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
LAMP2 / CD107b, Monoclonal Antibody (Cat# AAA249477)
Full Name
Anti-LAMP2 / CD107b Antibody IHC-plus
Gene Names
LAMP2; LAMPB; CD107b; LAMP-2; LGP110
Reactivity
Mouse, Human
Applications
IHC, IF, WB
Purity
Protein G purified
Pricing
$610/0.2 mL | $2,685/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-ITGA6/Integrin Alpha 6/CD49f antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
ITGA6/Integrin Alpha 6/CD49f, Monoclonal Antibody (Cat# AAA249173)
Full Name
Mouse Monoclonal (IgG1) to Human ITGA6/Integrin Alpha 6/CD49f
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human
Applications
IHC - Paraffin, IF, WB, FC/FACS
Purity
Protein G Purified
Pricing
$585/0.2 mL | $2,585/5x0.2 mL
Immunocytochemistry (ICC)
(Figure 1 Immunocytochemical staining of fiboblasts showing nuclear lamina)
Lamin A, Antibody (Cat# AAA570057)
Full Name
Mouse anti Lamin A
Gene Names
lmna; lmna-A
Reactivity
Bovine, Canine, Human, Mouse, Rat
Applications
FC, ICC, frozen, IHC-F, paraffin, IHC-P, WB
Pricing
$475/0.1 mg | $1,995/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). MBS9208263 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
FGFR2, Polyclonal Antibody (Cat# AAA9208263)
Full Name
FGFR2 Antibody (N-term)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, mouse, rat, monkey
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808968)
Full Name
Alpha Synuclein Antibody, Clone 3F8: RPE
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western Blot detection of human FOLR1 using MBS605669. Lysates were prepared from Hela cells in non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with MBS605669 (2ug/ml) and chemiluminescent detection substrate.)
FOLR1, Monoclonal Antibody (Cat# AAA605669)
Full Name
FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
$850/0.1 mg | $3,815/5x0.1 mg
Western Blot (WB)
(Western blot analysis of hsc70 using various lysates.Lanes 1 - 2: Merged signal (red and green). Green - AF5187 observed at 70kDa. Red - loading control, MBS9610328, observed at 55 kDa. Blots were developed with Goat Anti-Rabbit IgG(H+L)FITC–conjugated (MBS9612520) and Goat Anti-Mouse IgG(H+L) AlexaFluor 594–conjugated (MBS9612517) secondary antibodies)
beta-Tubulin, Monoclonal Antibody (Cat# AAA9610328)
Full Name
beta-Tubulin Mouse Monoclonal Antibody
Gene Names
TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat, Sheep, Rabbit, Dog, Monkey, Hamster, Chicken
Applications
WB, IHC, IF, ICC
Purity
Peptide affinity purification
Pricing
$190/0.05 mL | $245/0.1 mL | $310/0.2 mL | $855/1 mL | $3,800/5x1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808979)
Full Name
Alpha Synuclein Antibody, Clone 4F1: Alkaline Phosphatase
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808916)
Full Name
Alpha Synuclein Antibody, Clone 3C11
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human; Mouse; Rat
Applications
WB, DB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. stomach section using Pink1(115-213). MBS9200258 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
PINK1, Monoclonal Antibody (Cat# AAA9200258)
Full Name
PINK1 Antibody
Gene Names
PINK1; BRPK; PARK6
Reactivity
Human
Applications
EIA, IHC, IF, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of GFP using either natural fluorescence (green) or an GFP antibody (red) in Hela(human cervical epithelial adenocarcinoma cell line) cells transfected with GFP recombinant protein. Formalin-fixed cells were permeabilized with 0. 1% Triton X-100 for 10 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were probed with an GFP monoclonal antibody (Product # MBS9200313) at a dilution of 1:25 for 1 hour at 37°C, and incubated with DyLight 555 goat anti-mouse IgG secondary antibody (Product # please inquire) at a dilution of 1:200 for 60 minutes at 37°C. The nuclear counter stain is DAPI (blue).)
GFP Tag, Monoclonal Antibody (Cat# AAA9200313)
Full Name
GFP Tag Antibody
Reactivity
Human
Applications
IF, EIA, WB
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$190/0.05 mL | $435/0.2 mL | $1,705/5x0.2 mL
Immunohistochemistry (IHC)
(Anti-LAMP1 / CD107a antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
LAMP1 / CD107a, Monoclonal Antibody (Cat# AAA249200)
Full Name
Mouse Monoclonal [clone 6E2] (IgG1) to Human LAMP1 / CD107a
Gene Names
LAMP1; LAMPA; CD107a; LGP120
Reactivity
Bovine, Human, Mouse, Pig, Rat
Applications
IHC - Paraffin, ICC, IF, WB
Purity
Affinity Purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
KIT, Monoclonal Antibody (Cat# AAA9200373)
Full Name
KIT Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human
Applications
WB, EIA, IF
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC)
(Colorectal adenocarcinoma with diffuse strong expression of CK20 (A and B) and colorectal adenocarcinoma and normal epithelium of the large intestine mucosa with expression
of CK20 (C). Adenoma of the large intestine with expression of CK20 (D). All, formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti - Cytokeratin 20 (MBS684193) monospecific clonal antibody according to related datasheet.)
Cytokeratin 20, Monoclonal Antibody (Cat# AAA684193)
Full Name
Anti - Cytokeratin 20
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Applications
IHC
Pricing
$350/7 mL (RTU) | $330/0.1 mL (Concentrate) | $520/0.5 mL (Concentrate) | $780/1 mL (Concentrate) | $420/0.2 mL (Concentrate) | $485/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $3,245/5x1 mL (Concentrate)
Immunohistochemistry (IHC)
(Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
CD45, Monoclonal Antibody (Cat# AAA245401)
Full Name
Mouse Monoclonal [clone 3G4] (IgG2a) to Human CD45
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
IHC - Paraffin, WB, EIA
Purity
Protein G Purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Testing Data
Podoplanin, Monoclonal Antibody (Cat# AAA690143)
Full Name
Mouse Anti-Rat Podoplanin
Gene Names
Pdpn; E11; Gp38; OTS-8; RTI40; T1-alpha
Reactivity
ND
Applications
Western Blot, IHC/IF
Purity
Protein G purified
Pricing
$395/0.1 mg | $1,565/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808965)
Full Name
Alpha Synuclein Antibody, Clone 3F8: HRP
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3F8. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808967)
Full Name
Alpha Synuclein Antibody, Clone 3F8: PerCP
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using D14HD11 Cat.# GM-0505. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM1,3,4,5,6 (red curves) or an irrelevant protein (control transfectant: black curves). Binding of D14HD11 was detected with a PEconjugated secondary antibody. A positive signal was obtained only with CEACAM1, CEACAM3, CEACAM4, CEACAM5 and CEACAM6 expressing cells.)
CEACAM1,3,4,5,6, Monoclonal Antibody (Cat# AAA468008)
Full Name
anti-human CEACAM1,3,4,5,6 monoclonal antibody
Gene Names
CEACAM1; BGP; BGP1; BGPI
Reactivity
Human
Applications
FC/FACS, EIA, WB, IP, IF
Purity
Protein G
Pricing
$550/0.1 mg
Western Blot (WB)
(CD44 antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CD44 antibody detected the CD44 protein (arrow).)
CD44, Monoclonal Antibody (Cat# AAA9200576)
Full Name
CD44 Antibody
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human
Applications
WB, EIA, IHC, IF, FC/FACS
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Flow Cytometry (FC/FACS)
(Fig-1: Cell surface flow analysis of BST2 in PBMCs (lymphocytes gated) using 0.5 ug/10^6 cells of antibody (Clone: ABM52D8). Green represents isotype control; red represents anti-BST2 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice with PBS as mentioned above).)
BST 2, Monoclonal Antibody (Cat# AAA668285)
Full Name
Monoclonal Antibody to BST 2 (Clone: ABM52D8)
Gene Names
BST2; CD317; TETHERIN
Reactivity
Rat, Mouse, Human
Applications
FC/FACS, WB
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$325/0.1 mg | $165/0.025 mg | $1,405/5x0.1 mg
Immunohistochemistry (IHC)
(Fig-1: Immunohistochemical analysis of B7-H4 antibody in Renal cell carcinoma using anti-B7-H4 antibody (Clone: ABM53A6) at 5 ug/ml)
B7-H4, Monoclonal Antibody (Cat# AAA668282)
Full Name
Monoclonal Antibody to B7-H4 (Clone: ABM53A6)
Gene Names
VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
Reactivity
Mouse, Human
Applications
IHC, FC/FACS, WB
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$290/0.1 mg | $170/0.025 mg | $1,235/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Malondialdehyde, Monoclonal Antibody (Cat# AAA808772)
Full Name
Malondialdehyde Antibody, Clone 11E3
Reactivity
All
Applications
WB, ICC, IF, EIA, IHC
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Immunofluorescence (IF)
( Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody G4 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of G4 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
ED-B domain of fibronectin, Monoclonal Recombinant Antibody (Cat# AAA488256)
Full Name
Anti-ED-B domain of fibronectin [G4]
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
