Mouse LAMP1 / CD107a Monoclonal Antibody | anti-LAMP1 antibody

Mouse Monoclonal [clone 6E2] (IgG1) to Human LAMP1 / CD107a

Cat. Num. AAA249200

• Gene Names: LAMP1; LAMPA; CD107a; LGP120
• Reactivity: Bovine, Human, Mouse, Pig, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Affinity Purified
• Synonyms: LAMP1 / CD107a; Monoclonal Antibody; Mouse Monoclonal [clone 6E2] (IgG1) to Human LAMP1 / CD107a; Anti-LAMP1 / CD107a Antibody (clone 6E2) IHC-plus; LAMP1; CD107a; CD107a antigen; LAMP-1; LGP120; LAMPA; Human LAMP1; anti-LAMP1 antibody

• Host: Mouse
• Reactivity: Bovine, Human, Mouse, Pig, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6E2
• Purity/Purification: Affinity Purified
• Form/Format: PBS, 10 mM sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 417

• Applicable Applications for anti-LAMP1 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IHC-Paraffin (1:50); ICC (1:2000); Immunofluorescence (1:2000); Western Blot (1:10000); (Optimal dilution to be determined by the researcher); ; Usage: The antibody solution can be used at dilutions of at least 1:2000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10000 or lower.
• Immunogen Species: Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-LAMP1 / CD107a antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-LAMP1 / CD107a antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Immunocytochemistry (ICC)

(HeLa cells staining with CD107a / LAMP1 antibody (green), and counterstained with chicken polyclonal antibody to Lamin A/C (red) and DNA (blue). The CD107a / LAMP1 antibody antibody reveals strong cytoplasmic staining of lysosomes and early endosomes, while the Lamin A/C antibody binds to the nuclear lamina. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.)

Western Blot (WB)

(Western strip blots of HeLa cell crude extracts with two different preparations of CD107a / LAMP1 antibody in strips 17 and 18. Both preparations bind to a diffuse band running at between ~90kDa and ~120kDa as expected, and show no appreciably cross reactivity with any other protein.)

Related Product Information for anti-LAMP1 antibody

LAMP1, also known as CD107a, is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP1 may also play a role in tumor cell metastasis. It has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells.

NCBI and Uniprot Product Information

• NCBI GI #: 112380628
• NCBI GeneID: 3916
• NCBI Accession #: NP_005552.3
• NCBI GenBank Nucleotide #: NM_005561.3
• UniProt Accession #: P11279; Q8WU33; Q96I40; Q9BRD2; Q9NP13; B4DWL3
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 1
• NCBI Official Synonym Full Names: lysosomal-associated membrane protein 1
• NCBI Official Symbol: LAMP1
• NCBI Official Synonym Symbols: LAMPA; CD107a; LGP120
• NCBI Protein Information: lysosome-associated membrane glycoprotein 1; LAMP-1; CD107 antigen-like family member A; lysosome-associated membrane protein 1
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 1
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: LAMP1
• UniProt Synonym Gene Names: LAMP-1; Lysosome-associated membrane protein 1
• UniProt Entry Name: LAMP1_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMP1: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. Belongs to the LAMP family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: lysosome; integral to plasma membrane; dendrite; multivesicular body; membrane; cell soma; cytoplasm; late endosome; melanosome; plasma membrane; endosome membrane; sarcolemma; external side of plasma membrane

Molecular Function: protein domain specific binding; protein binding; enzyme binding

Biological Process: positive regulation of natural killer cell degranulation; induction of apoptosis by granzyme; protein stabilization; viral reproduction; autophagic cell death; autophagy; positive regulation of natural killer cell mediated cytotoxicity