Immunohistochemistry (IHC)
(Fig-1: Immunohistochemical analysis of B7-H4 antibody in Renal cell carcinoma using anti-B7-H4 antibody (Clone: ABM53A6) at 5 ug/ml)
Mouse anti-Mouse, Human B7-H4 Monoclonal Antibody | anti-B7-H4 antibody
Monoclonal Antibody to B7-H4 (Clone: ABM53A6)
Protein G Chromatography
Protein G Chromatography
IHC: 5 ug/ml
WB: 2-4 ug/ml
For long-term storage, store at -20°C.
Avoid repeated freeze and thaw cycles.
Immunohistochemistry (IHC)
(Fig-1: Immunohistochemical analysis of B7-H4 antibody in Renal cell carcinoma using anti-B7-H4 antibody (Clone: ABM53A6) at 5 ug/ml)
Immunohistochemistry (IHC)
(Fig-1: Immunohistochemical analysis of B7-H4 antibody in Renal cell carcinoma using anti-B7-H4 antibody (Clone: ABM53A6) at 5 ug/ml)
Flow Cytometry (FC/FACS)
(Fig-2: Cell surface Flow analysis of B7-H4 antibody in human PBMC (Monocytes) cells using 0.5 ug/ 10^6 cells of anti-B7-H4 antibody (ABM53A6). Green represents isotype control;red represents anti-B7H4 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
Flow Cytometry (FC/FACS)
(Fig-2: Cell surface Flow analysis of B7-H4 antibody in human PBMC (Monocytes) cells using 0.5 ug/ 10^6 cells of anti-B7-H4 antibody (ABM53A6). Green represents isotype control;red represents anti-B7H4 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
Western Blot (WB)
(Fig-3: Western blot analysis of B7H4. Anti-B7H4 antibody (Clone: ABM53A6) was used at 4 ug/ml on human B7H4-FC fusion protein Lysate.)
Western Blot (WB)
(Fig-3: Western blot analysis of B7H4. Anti-B7H4 antibody (Clone: ABM53A6) was used at 4 ug/ml on human B7H4-FC fusion protein Lysate.)
Western Blot(WB)
(Fig-4: Western blot analysis of B7-H4. Anti-B7-H4 antibody (Clone: ABM53A6) was used at 2 ug/ml on (1) HCT-116, (2) PC3, (3) Kato 111, (4) C2C12 and (5) RAW Lysates.)
Western Blot(WB)
(Fig-4: Western blot analysis of B7-H4. Anti-B7-H4 antibody (Clone: ABM53A6) was used at 2 ug/ml on (1) HCT-116, (2) PC3, (3) Kato 111, (4) C2C12 and (5) RAW Lysates.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p13.1-p12
Research Articles on B7-H4
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Product Notes
The B7-H4 vtcn1 (Catalog #AAA668282) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to B7-H4 (Clone: ABM53A6) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). FACS: 0.5-1 ug/10^6 cells IHC: 5 ug/ml WB: 2-4 ug/ml. Researchers should empirically determine the suitability of the B7-H4 vtcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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