Rabbit NFkB, p65 Monoclonal Antibody | anti-NKIRAS2 antibody

NFkB, p65, phosphorylated (Ser536) (NF Kappa B, NF-kB, Nuclear Factor kappa B)

Cat. Num. AAA633051

• Gene Names: NKIRAS2; KBRAS2; kappaB-Ras2
• Reactivity: Hamster, Human, Monkey, Mouse, Porcine, Rat
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: NFkB; p65; Monoclonal Antibody; phosphorylated (Ser536) (NF Kappa B; NF-kB; Nuclear Factor kappa B); Anti -NFkB; anti-NKIRAS2 antibody

• Host: Rabbit
• Reactivity: Hamster, Human, Monkey, Mouse, Porcine, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 4i11
• Specificity: Detects NF-kB p65 only when phosphorylated at serine 536. Does not crossreact with the p50 subunit or other related proteins. Species Crossreactivity
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-NKIRAS2 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry.; Dilution: Note: Anti-rabbit secondary antibodies must be used to detect N2303-01B; Western Blot: 1:1,000; Immunoprecipitation: 1:50; Immunofluorescence: 1:100; Flow Cytometry: 1:1,600
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kB p65 (KLH-coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TNF-alpha treated, using using MBS633051, NFkB p65, phosphorylated antibody (upper) or N2302-04, NFkB p65 antibody (lower).)

Western Blot (WB)

(Western Blot analysis of extracts from HeLa cells treated with TNF-a or calf intestinal phosphatase (CIP), using MBS633051, NFkB p65, phosphorylated antibody (upper) or N2302-04, NFkB p65 antibody (lower).)

Related Product Information for anti-NKIRAS2 antibody

Transcription factors of the nuclear factor kB (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). In mammals the family, which is comprised of five proteins, Rel-A, c-Rel, Rel-B, NF-kB1 (p105/p50), and NF-kB2 (p100/p52). p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. The p50 and p52 products form dimeric complexes with Rel proteins, which are then able to bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by its inhibitory proteins, the IkBs (3-5). NF-kB-activating agents can induce the phosphorylation of IkBs, which targets them for rapid degradation through a ubiquitin-proteasome pathway, releasing NF-kB to enter the nucleus and regulate gene expression (6-8). Processing of NF-kB2 is regulated by IKK1 (IKK-a), which triggers the phosphorylation and processing to p52, which can then undergo nuclear translocation (9-11).

Product Categories/Family for anti-NKIRAS2 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 222136632
• NCBI GeneID: 28511
• NCBI Accession #: NP_001138401.1
• NCBI GenBank Nucleotide #: NM_001144929.1
• UniProt Accession #: Q9NYR9; Q6PK52; Q96KC7; Q9NSX1; A6NCZ5; B3KNN0; B4DNM3
• Molecular Weight: 21,508 Da
• NCBI Official Full Name: NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform c
• NCBI Official Synonym Full Names: NFKB inhibitor interacting Ras-like 2
• NCBI Official Symbol: NKIRAS2
• NCBI Official Synonym Symbols: KBRAS2; kappaB-Ras2
• NCBI Protein Information: NF-kappa-B inhibitor-interacting Ras-like protein 2; kappa B-Ras protein 2; I-kappa-B-interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like protein 2
• UniProt Protein Name: NF-kappa-B inhibitor-interacting Ras-like protein 2
• Protein Family: NF-kappa-B inhibitor-interacting Ras-like protein
• UniProt Gene Name: NKIRAS2
• UniProt Synonym Gene Names: KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2
• UniProt Entry Name: KBRS2_HUMAN

Uniprot Description

NKIRAS2: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF- kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Ras; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: membrane; cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction