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Western Blot (WB)
(All lanes : Anti-Ctr9 Antibody (C-term) at 1:1000 dilutionLane 1: HT-29 whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: mouse cerebellum lysatesLane 4: mouse testis lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 133 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse Ctr9 Polyclonal Antibody | anti-Ctr9 antibody
Mouse Ctr9 Antibody (C-term)
Gene Names
Ctr9; Tsp; Tsbp; Sh2bp1; AA409336; mKIAA0155
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Synonyms
Ctr9; Polyclonal Antibody; Mouse Ctr9 Antibody (C-term); RNA polymerase-associated protein CTR9 homolog; SH2 domain-binding protein 1; Tetratricopeptide repeat-containing; SH2-binding phosphoprotein of 150 kDa; TPR-containing; p150TSP; Kiaa0155; Sh2bp1; anti-Ctr9 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This Mouse Ctr9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1022-1056 amino acids from the C-terminal region of Mouse Ctr9.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
1173
Applicable Applications for anti-Ctr9 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:1000
IHC-P: 1:25
IF: 1:25
IHC-P: 1:25
IF: 1:25
Antigen Source
Human
Antigen Region
1022-1056
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-Ctr9 Antibody (C-term) at 1:1000 dilutionLane 1: HT-29 whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: mouse cerebellum lysatesLane 4: mouse testis lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 133 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Ctr9 Antibody (C-term) at 1:1000 dilutionLane 1: HT-29 whole cell lysatesLane 2: Jurkat whole cell lysatesLane 3: mouse cerebellum lysatesLane 4: mouse testis lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 133 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded M. testis section using Mouse Ctr9 Antibody (C-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded M. testis section using Mouse Ctr9 Antibody (C-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded M. colon section using Mouse Ctr9 Antibody (C-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)-Paraffin
(Immunohistochemical analysis of paraffin-embedded M. colon section using Mouse Ctr9 Antibody (C-term) . It was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunofluorescence (IF)
(Fluorescent image of A431 cells stained with Mouse Ctr9 Antibody (C-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of A431 cells stained with Mouse Ctr9 Antibody (C-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-Ctr9 antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Required for mono- and trimethylation on histone H3 'Lys- 4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription (By similarity). Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3.
Background: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Required for mono- and trimethylation on histone H3 'Lys- 4' (H3K4me3) and dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription (By similarity). Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of the SET1 complex. Involved in transcriptional regulation of IL6-responsive genes and in JAK-STAT pathway; may regulate DNA-association of STAT3.
References
Malek S.N.,et al.J. Biol. Chem. 271:6952-6962(1996). Carninci P.,et al.Science 309:1559-1563(2005). Okazaki N.,et al.DNA Res. 9:179-188(2002). Ballif B.A.,et al.Mol. Cell. Proteomics 3:1093-1101(2004). Youn M.Y.,et al.J. Biol. Chem. 282:34727-34734(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133408
NCBI Official Full Name
RNA polymerase-associated protein CTR9 homolog
NCBI Official Synonym Full Names
CTR9 homolog, Paf1/RNA polymerase II complex component
NCBI Official Symbol
Ctr9
NCBI Official Synonym Symbols
Tsp; Tsbp; Sh2bp1; AA409336; mKIAA0155
NCBI Protein Information
RNA polymerase-associated protein CTR9 homolog
UniProt Protein Name
RNA polymerase-associated protein CTR9 homolog
Protein Family
UniProt Gene Name
Ctr9
UniProt Synonym Gene Names
Kiaa0155; Sh2bp1; TPR-containing, SH2-binding phosphoprotein of 150 kDa; p150TSP
UniProt Entry Name
CTR9_MOUSE
Research Articles on Ctr9
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Product Notes
The Ctr9 ctr9 (Catalog #AAA9231990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Ctr9 Antibody (C-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ctr9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB: 1:1000 IHC-P: 1:25 IF: 1:25. Researchers should empirically determine the suitability of the Ctr9 ctr9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ctr9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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