Testing Data
Rabbit anti-Human CD 20 Monoclonal Antibody | anti-CD 20 antibody
Anti - CD 20
2.Wash the section in 96%, 80% and 70% thyl alcohol for 10 minutes each. .
3.Rinse in distilled water. .
4.Block the endogenous peroxidase by incubating the tissuein 3% hydrogen peroxide (H2O2) for 10 minutes. .
5.Wash in distilled water for 5 minutes. .
6.For antigen retrieval: immerse the slide in the citrate buffer, pH 6.0, 0.05% Tween-20*, and incubate in ater bath at 96-98°C for 25 minutes. (Alternatively adjust to your own protocol, keeping the required pH) .
7.Transfer the slide to room temperature and let it cool down (in citrate buffer, pH 6.0)for 15 minutes.
8.Rinse in distilled water. .
9.Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.
10.CONCENTRATED:Incubate the section with primary antibody at the dilution 1:100 -1:200 for 1 hour in the closed wet chamber. .
.READY TO USE (RTU):Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber.
11.Wash twice 5 minutes with buffer A. .
12.Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP -Peroxide -DAB). Micropolymer-HRP detection kit rabbit/mouse .
13.Wash twice 5 minutes with buffer A. .
14.Apply the chromogen (DAB), 1 -3 minutes. .
15.Wash in water for 10 minutes. .
16.Stain in hematoxylin for 5 minutes. .
17.Wash in water for 10 minutes. .
18.Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.
19.Wash the section in 2 changes of xylene for 2 minutes each. .
20.Mount the slide for observation.
Citrate Buffer(10mM Citric Acid, 0.05% Tween-20, pH 6.0):
Citric acid (anhydrous) ----------1.92 g; Distilled water ---------1000 ml.
Mix to dissolve in 700 ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5 ml of Tween-20 and mix well.
Adjust the final volume to 1 liter with distilled water.
Store this solution at room temperature for 3 months or at +4°C for longer storage.
2.Centrifuge the vial before use.
3.Intended for professional use in laboratories. .
4.Do not use after expiration date stamped on vial label. .
5.Avoid contamination of the reagent. .
6.Any discrepancies in the recommended procedures stated in the working protocol may affect the final results. .
7.The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous. .
8.Disposal of waste material must be conducted in accordance with local regulations. .
9.Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: This protein may be involved in the regulation of B-cell activation and proliferation.
Subcellular location: Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor Ref.11.
Tissue specificity: Expressed on B-cells.
Post-translational modification: Phosphorylated. Might be functionally regulated by protein kinase(s).
Involvement in disease: Immunodeficiency, common variable, 5 (CVID5) [MIM:613495]: A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10
Pharmaceutical use: Monoclonal antibodies (mAb) against CD20 are used to treat B-cell non-Hodgkin lymphoma (NHL). These antibodies include Rituximab (Mabthera), Britumomab (Zevalin) and Tositumomab (Bexxar). CD20 engaged by mAb can generate transmembrane signals capable of directly controlling cell growth and triggering cell death in certain tumors. Alternatively, mAb can mediate complement-dependent cytotoxicity.
Sequence similarities: Belongs to the MS4A family.
Caution: Epitope 1, mapped in Ref.8, is predicted to be buried in the membrane. Its accessibility to the extracellular space, and thus to antibody recognition, is not explained.
Research Articles on CD 20
Similar Products
Product Notes
The CD 20 ms4a1 (Catalog #AAA684044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - CD 20 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD 20 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) - Formalin/Paraffin. IHC-P dilution 1:100 - 1:200. Researchers should empirically determine the suitability of the CD 20 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD 20, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
