Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Filters


Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

10000 results for " T Cell" - showing 4150-4200


Testing Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)

CD3, Monoclonal Antibody (Cat# AAA215211)

Full Name
MOUSE ANTI DOG CD3
Applications
FC/FACS, IF, IP
Pricing
$395/0.1 mg | $1,630/5x0.1 mg

CD2, Polyclonal Antibody (Cat# AAA541197)

Full Name
CD2 Antibody FITC-Conjugated
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human, Monkey
Predicted: Mouse, Rat
Applications
EIA, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)

cadherin 13, H-cadherin (heart), ELISA Kit (Cat# AAA703388)

Full Name
Human H-Cadherin, H-Cad/CDH13 ELISA Kit
Gene Names
CDH13; CDHH; P105
Reactivity
Human
Pricing
$590/48-Strip-Wells | $845/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $3,055/5x96-Strip-Wells | $5,830/10x96-Strip-Wells
Testing Data

CD8a (Ly 2), Monoclonal Antibody (Cat# AAA520215)

Full Name
Anti-Mouse CD8a (Ly 2) Purified (Clone CT-CD8a) (rat IgG2a)
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Mouse
Applications
F, C, IV, P
Pricing
$495/0.2 mg | $2,000/5x0.2 mg
Testing Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD8)

CD8, Monoclonal Antibody (Cat# AAA212993)

Full Name
RAT ANTI HUMAN CD8
Gene Names
CD8A; CD8; MAL; p32; Leu2
Applications
FC, IF
Pricing
$475/0.2 mg | $1,995/5x0.2 mg

CD2, Recombinant Protein (Cat# AAA636923)

Full Name
CD2, Recombinant, Human (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2)
Gene Names
CD2; T11; SRBC; LFA-2
Applications
EL/EIA, WB
Purity
Purified
On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 46kD
Pricing
$515/0.002 mg | $625/0.005 mg | $2,795/5x0.005 mg

soluble CD40 Ligand/TRAP, Recombinant Protein (Cat# AAA143564)

Full Name
Recombinant Human soluble CD40 Ligand/TRAP His Tag
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
$250/0.02 mg | $2,830/1 mg | $180/0.005 mg | $12,520/5x1 mg
Western Blot (WB) ()

14-3-3 beta, Monoclonal Antibody (Cat# AAA834603)

Full Name
14-3-3 beta antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Applications
WB
Purity
14-3-3 beta antibody was purified by ammonium sulfate precipitation
Testing Data

CD5 (Ly 1.2), Monoclonal Antibody (Cat# AAA520184)

Full Name
Anti-Mouse CD5 (Ly 1.2), PE (Clone CG16) (mouse IgG2b)
Gene Names
CD5; T1; LEU1
Reactivity
Mouse
Applications
IHC
Pricing
$255/0.05 mg | $575/0.3 mg | $2,375/5x0.3 mg
Testing Data #1 (Western Blot analysis of HeLa whole cell lysate probed with Rabbit anti Human CD184 (MBS221580))

CD184, Polyclonal Antibody (Cat# AAA221580)

Full Name
RABBIT ANTI HUMAN CD184 (N-TERMINAL)
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E
Applications
FC/FACS, IP, WB
Pricing
$420/0.1 mg | $1,735/5x0.1 mg

T-cell acute lymphoblastic leukemia Antigen (TALLA-1), ELISA Kit (Cat# AAA2609834)

Full Name
Bovine T-cell acute lymphoblastic leukemia Antigen(TALLA-1) ELISA Kit
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Bovine
Pricing
$300/48-Strip-Wells | $465/96-Strip-Wells | $1,930/5x96-Strip-Wells | $3,535/10x96-Strip-Wells
SDS-PAGE

T-box transcription factor TBX18, Recombinant Protein (Cat# AAA1078272)

Full Name
Recombinant Human T-box transcription factor TBX18
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,415/1 mg (E-Coli) | $395/0.1 mg (E-Coli) | $245/0.02 mg (E-Coli) | $6,205/5x1 mg (E-Coli)

(Rhesus macaque) CD226 antigen (CD226), Recombinant Protein (Cat# AAA1309512)

Full Name
Recombinant Macaca mulatta (Rhesus macaque) CD226 antigen (CD226)
Gene Names
CD226; PTA1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Typical Testing Data/Standard Curve (for reference only)

Protectin, ELISA Kit (Cat# AAA730752)

Full Name
Human Protectin ELISA Kit
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human
Pricing
$475/48-Strip-Wells-(Competitive) | $680/96-Strip-Wells-(Competitive) | $475/48-Strip-Wells-(Sandwich) | $680/96-Strip-Wells-(Sandwich) | $3,010/5x96-Strip-Wells-(Competitive) | $5,675/10x96-Strip-Wells-(Competitive) | $3,010/5x96-Strip-Wells-(Sandwich) | $5,675/10x96-Strip-Wells-(Sandwich)
Typical Testing Data/Standard Curve (for reference only)

bone morphogenetic protein receptor, type II (serine/threonine kinase), ELISA Kit (Cat# AAA703759)

Full Name
Human Bone morphogenetic protein receptor II, BMPR-II ELISA Kit
Gene Names
BMPR2; BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
Reactivity
Human
Pricing
$530/48-Strip-Wells | $755/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,875/5x96-Strip-Wells | $5,480/10x96-Strip-Wells
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human Tonsil Caricnoma using CD2 Mouse mAb diluted at 1:500.)

CD2, Monoclonal Antibody (Cat# AAA9417443)

Full Name
CD2 Monoclonal Antibody(3F3)
Reactivity
Human, Mouse, Rat
Applications
IHC
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Testing Data

CD59, Monoclonal Antibody (Cat# AAA520190)

Full Name
Anti-Human CD59, (FITC) (Clone 1F5) (mouse IgG1)
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human
Applications
IHC
Pricing
$295/0.1 mg | $1,105/5x0.1 mg

TIM3, Monoclonal Antibody (Cat# AAA520440)

Full Name
Anti-Mouse TIM3, FITC, (clone: 25F.1D6), (rat IgG2ak)
Reactivity
Mouse
Applications
IHC
Pricing
$310/0.1 mg | $1,170/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)

IL-6, ELISA Kit (Cat# AAA175908)

Full Name
Rat IL-6 PicoKine ELISA Kit
Gene Names
Il6; ILg6; Ifnb2
Reactivity
Rat
Pricing
$470/96-Strip-Wells | $2,110/5x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)

Immunoglobulin G4 (IgG4), ELISA Kit (Cat# AAA703648)

Full Name
Human Immunoglobulin G4 (IgG4) ELISA Kit
Gene Names
ICOS; AILIM; CD278; CVID1
Reactivity
Human
Pricing
$470/48-Strip-Wells | $665/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,750/5x96-Strip-Wells | $5,230/10x96-Strip-Wells

CD8a, Monoclonal Antibody (Cat# AAA532681)

Full Name
CD8a antibody (biotin)
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Chicken
Applications
FC/FACS, IHC, IP
Pricing
$615/0.5 mg | $2,755/5x0.5 mg
Typical Testing Data/Standard Curve (for reference only)

inducible T-cell co-stimulator ligand, ELISA Kit (Cat# AAA927767)

Full Name
Human ICOS ligand, ICOSLG ELISA Kit
Gene Names
ICOSLG; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Reactivity
Human
Pricing
$530/48-Strip-Wells | $755/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,875/5x96-Strip-Wells | $5,480/10x96-Strip-Wells

CD4/L3T4, Monoclonal Antibody (Cat# AAA673155)

Full Name
Rat Anti-Mouse CD4/L3T4-FITC
Gene Names
Cd4; L3T4; Ly-4
Reactivity
Mouse
Applications
Flow Cytometry; Immunohistochemistry; Immunoprecipitation
Pricing
$265/0.5 mg | $1,040/5x0.5 mg

CD8a, Monoclonal Antibody (Cat# AAA532685)

Full Name
CD8a antibody (biotin)
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Chicken
Applications
FC/FACS, IHC, IP
Pricing
$615/0.5 mg | $2,765/5x0.5 mg
Western Blot (WB) ((0.5ug/ml) staining of Human Tonsil (A) and Mouse Kidney (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)

GATA3, Polyclonal Antibody (Cat# AAA421671)

Full Name
Goat anti-GATA3 Antibody
Gene Names
GATA3; HDR; HDRS
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
EIA, WB, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg

CD2, Polyclonal Antibody (Cat# AAA541196)

Full Name
CD2 Antibody BIOTIN-Conjugated
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human, Monkey
Predicted:Mouse, Rat
Applications
EIA, IHC, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Immunohistochemistry (IHC) (Anti-CD8A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:25.)

CD8A / CD8 Alpha, Polyclonal Antibody (Cat# AAA247657)

Full Name
Rabbit Polyclonal [clone P17-V] to Human CD8A / CD8 Alpha
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Human
Applications
IHC - Paraffin; IHC - Frozen
Purity
Purified
Pricing
$585/0.125 mL | $2,585/5x0.125 mL

CD2 - CD20, Antibody (Cat# AAA396183)

Full Name
CD2 FITC - CD20 PE
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human
Applications
FC/FACS
Purity
Protein A/G Chromatography
Pricing
$450/100 Tests | $1,870/5x100 Tests
Typical Testing Data/Standard Curve (for reference only)

Lymphocyte Antigen 9 (LY9), ELISA Kit (Cat# AAA2020709)

Full Name
Human Lymphocyte Antigen 9 (LY9) ELISA Kit
Gene Names
LY9; hly9; mLY9; CD229; SLAMF3
Reactivity
Human
Pricing
$510/48-Strip-Wells | $685/96-Strip-Wells | $2,745/5x96-Strip-Wells | $5,005/10x96-Strip-Wells | $340/24-Strip-Wells
Testing Data

CD8a (Ly 2.1), Monoclonal Antibody (Cat# AAA520217)

Full Name
Anti-Mouse CD8a (Ly 2.1), Biotin (Clone 49-31.1) (mouse IgG3)
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Mouse
Applications
IHC
Pricing
$250/0.1 mg | $435/0.3 mg | $1,730/5x0.3 mg

Fas, Neutralizing, Monoclonal Antibody (Cat# AAA601701)

Full Name
Fas, Neutralizing (CD95, APO-1)
Reactivity
Human
Applications
WB, FC/FACS
Purity
Affinity Purified
Purified by ammonium sulfate precipitation and Protein A affinity chromatography.
Pricing
$870/0.1 mg | $3,910/5x0.1 mg

CD40L, Monoclonal Antibody (Cat# AAA312100)

Full Name
MAb to CD40L
Gene Names
CD40LG; IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Reactivity
Human
Applications
EIA/ELISA, Immunoassay Antibody Pairs
Purity
>90% pure. Protein A Sepharose chromatography. Purity is tested by electrophoresis.
Pricing
$760/1 mg | $3,385/5x1 mg

AITRL, Active Protein (Cat# AAA539604)

Full Name
AITRL protein
Applications
User optimized
Purity
> 98% pure
Pricing
$425/0.02 mg | $1,905/5x0.02 mg
Flow Cytometry (FC/FACS) (Staining of normal human peripheral blood cells with CD45RA antibody and CD45RO antibody (PE). Cells in the lymphocyte gate were used for analysis.)

CD45RO, Monoclonal Antibody (Cat# AAA833108)

Full Name
CD45RO antibody (PE)
Reactivity
Human CD45RO
Applications
Flow Cytometric Analysis
Pricing
$640/100 Tests | $2,880/5x100 Tests

SNL Feeder Cells, Cell Line (Cat# AAA169018)

Full Name
SNL Feeder Cells
Pricing
$810/1 Vial | $3,450/5x1 Vial
Western Blot (WB) (Western Blot analysis using MBS602684 (1:1000)|Lane 1: Human Interleukin 23, p40 fusion protein|Lane 2: Human Interleukin 23, p19 fusion protein|Lane 3: Recombinant human single chain Interleukin 23 (DYKDDDDK-tagged))

Interleukin 23, p40, Monoclonal Antibody (Cat# AAA602684)

Full Name
Interleukin 23, p40 (IL-23)
Gene Names
IL23A; P19; SGRF; IL-23; IL-23A; IL23P19
Reactivity
Human
Applications
EL/EIA, WB
Purity
Purified
Purified
Pricing
$560/0.05 mg | $2,515/5x0.05 mg
Immunohistochemistry (IHC) (Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

CCL5 / RANTES, Monoclonal Antibody (Cat# AAA245262)

Full Name
Mouse Monoclonal [clone 8.PP.11] (IgG2b) to Human CCL5 / RANTES
Gene Names
CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
Reactivity
Human
Applications
IHC - Paraffin, EIA
Purity
Protein A Purified
Pricing
$585/0.05 mg | $2,585/5x0.05 mg

YWHAQ, Recombinant Protein (Cat# AAA286143)

Full Name
Recombinant Human YWHAQ Protein
Gene Names
YWHAQ; 1C5; HS1; 14-3-3
Applications
WB, EIA
Purity
> 95% as determined by SDS-PAGE
Pricing
$440/0.1 mg | $1,220/0.5 mg | $1,695/1 mg | $7,630/5x1 mg

CD8a, Monoclonal Antibody (Cat# AAA532674)

Full Name
CD8a antibody (biotin)
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Pig
Applications
FC/FACS, IHC, IP
Pricing
$725/0.5 mg | $3,260/5x0.5 mg
Typical Testing Data/Standard Curve (for reference only)

CD59 molecule, complement regulatory protein, ELISA Kit (Cat# AAA900882)

Full Name
Human CD59 glycoprotein, CD59 ELISA Kit
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human
Pricing
$530/48-Strip-Wells | $755/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,875/5x96-Strip-Wells | $5,480/10x96-Strip-Wells
Flow Cytometry (Mouse Strain: BALB/c Cell Concentration : 1x10^6 cells per test Antibody Concentration Used: 0.1 ug/10^6 cells Isotypic Control: Rat IgG2b Cell Source/ Percentage of cells stained above control: Bone Marrow Macrophages/ 79.5% Peritoneal Macrophages/ 44.6% Thymus/ 0.7% Cell Source: Peritoneal Macrophages Percentage of cells stained above control: 44.6%)

CD11b, Monoclonal Antibody (Cat# AAA520040)

Full Name
Anti-Mouse CD11b, Purified (Clone M1/70.15) (rat IgG2b)
Gene Names
ITGAM; CD11B
Reactivity
Mouse
Applications
IHC, FC/FACS, EIA, WB, IP, FA
Pricing
$310/0.25 mg | $1,165/5x0.25 mg

APRIL (ED), Polyclonal Antibody (Cat# AAA194153)

Full Name
APRIL (ED) Polyclonal Antibody
Applications
Immunoblot
Pricing
$475/0.1 mg | $2,090/5x0.1 mg

CD8a, Monoclonal Antibody (Cat# AAA532689)

Full Name
CD8a antibody (biotin)
Gene Names
CD8A; CD8; MAL; p32; Leu2
Reactivity
Chicken
Applications
FC/FACS, IHC, IP
Pricing
$615/0.5 mg | $2,765/5x0.5 mg
Testing Data (Staining formalin-fixed, paraffin-embedded human tonsil with Mouse anti Human CD19)

CD19, Monoclonal Antibody (Cat# AAA212806)

Full Name
MOUSE ANTI HUMAN CD19
Gene Names
CD19; B4; CVID3
Applications
EIA, FC/FACS, IP, WB
Pricing
$525/0.2 mg | $2,210/5x0.2 mg
SDS-PAGE

T cell receptor alpha constant (TRAC), Recombinant Protein (Cat# AAA957674)

Full Name
Recombinant Human T cell receptor alpha constant (TRAC)
Gene Names
TRAC; TRA; IMD7; TCRA; TRCA
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,415/1 mg (E-Coli) | $1,055/0.5 mg (Yeast) | $395/0.1 mg (E-Coli) | $440/0.1 mg (Yeast) | $245/0.02 mg (E-Coli) | $260/0.02 mg (Yeast) | $6,205/5x1 mg (E-Coli) | $4,520/5x0.5 mg (Yeast)
SDS-PAGE

DNA mismatch repair protein Msh6, Recombinant Protein (Cat# AAA957892)

Full Name
Recombinant Human DNA mismatch repair protein Msh6
Gene Names
MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,415/1 mg (E-Coli) | $395/0.1 mg (E-Coli) | $245/0.02 mg (E-Coli) | $6,205/5x1 mg (E-Coli)
Immunohistochemistry (IHC) (P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Abl1 (phospho-Tyr412) antibody.)

Abl1, Antibody (Cat# AAA857515)

Full Name
Abl1 (Phospho-Tyr412) Antibody
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; bcr/abl
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) ((0. 03ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

14-3-3 tau/YWHAQ, Polyclonal Antibody (Cat# AAA420856)

Full Name
Goat anti-14-3-3 tau/YWHAQ Antibody
Gene Names
YWHAQ; 1C5; HS1; 14-3-3
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg

CD40, Recombinant Protein (Cat# AAA538087)

Full Name
CD40 protein
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Applications
User optimized
Purity
> 98% pure
Pricing
$425/0.05 mg | $1,905/5x0.05 mg
SDS-Page

TCL1A, Recombinant Protein (Cat# AAA203392)

Full Name
TCL1A, 1-114aa, Human, E Coli
Gene Names
TCL1A; TCL1
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
$190/0.02 mg | $280/0.1 mg | $570/0.5 mg | $2,325/5x0.5 mg
Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.