Human cadherin 13, H-cadherin (heart) ELISA Kit | CDH13 elisa kit

Human H-Cadherin, H-Cad/CDH13 ELISA Kit

Cat. Num. AAA703388

• Gene Names: CDH13; CDHH; P105
• Reactivity: Human
• Synonyms: cadherin 13; H-cadherin (heart); Human H-Cadherin; H-Cad/CDH13 ELISA Kit; CDHH; H-cadherin; P105; T-cad; T-cadherin; heart-cadherin; truncated-cadherin; CDH13 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CDH13. No significant cross-reactivity or interference between Human CDH13 and analogues was observed.
• Sequence Length: 674

• Samples: Serum, plasma and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 1.25 ng/ml-80 ng/ml
• Sensitivity: 0.312 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cell Adhesion
• Protein Biological Process 3: Cell adhesion
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDH13 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for H-Cad/CDH13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any H-Cad/CDH13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for H-Cad/CDH13 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of H-Cad/CDH13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 333944018
• NCBI GeneID: 1012
• NCBI Accession #: NP_001207418.1
• NCBI GenBank Nucleotide #: NM_001220489.1
• UniProt Accession #: P55290; Q6GTW4; Q8TBX3; A8W476; A8W477; B7Z590; C9JRI6; J3KN62
• Molecular Weight: 78,287 Da
• NCBI Official Full Name: cadherin-13 isoform 3
• NCBI Official Synonym Full Names: cadherin 13
• NCBI Official Symbol: CDH13
• NCBI Official Synonym Symbols: CDHH; P105
• NCBI Protein Information: cadherin-13; T-cad; T-cadherin; heart cadherin; H-cadherin (heart); cadherin 13, H-cadherin (heart)
• UniProt Protein Name: Cadherin-13
• Protein Family: Cadherin
• UniProt Gene Name: CDH13
• UniProt Synonym Gene Names: CDHH; H-cadherin; T-cad; T-cadherin
• UniProt Entry Name: CAD13_HUMAN

NCBI Description

This gene encodes a member of the cadherin superfamily. The encoded protein is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. The protein lacks the cytoplasmic domain characteristic of other cadherins, and so is not thought to be a cell-cell adhesion glycoprotein. This protein acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. The gene is hypermethylated in many types of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2011]

Uniprot Description

Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.1 PublicationManual assertion based on experiment in:Ref.9