Typical Testing Data/Standard Curve (for reference only)
Mouse Aspartate Aminotransferase (AST) ELISA Kit | AST elisa kit
Mini Samples Aspartate Aminotransferase (AST) ELISA Kit
Gene Names
Got1; cCAT; Got-1; cAspAT; AI789014
Reactivity
Mouse
Applications
ELISA
Synonyms
Aspartate Aminotransferase (AST); Mini Samples Aspartate Aminotransferase (AST) ELISA Kit; cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase; cytoplasmic; Transaminase A; Aspartate Transaminase 1; Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1; Soluble; AST elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of aspartate aminotransferase. No significant cross-reactivity or interference between aspartate aminotransferase and analogues was observed.
Applicable Applications for AST elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
< 0.112ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level aspartate aminotransferase were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level aspartate aminotransferase were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AST elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of aspartate aminotransferase in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to aspartate aminotransferase. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to aspartate aminotransferase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain aspartate aminotransferase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of aspartate aminotransferase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to aspartate aminotransferase. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to aspartate aminotransferase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain aspartate aminotransferase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of aspartate aminotransferase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,248 Da
NCBI Official Full Name
aspartate aminotransferase, cytoplasmic
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 1, soluble
NCBI Official Symbol
Got1
NCBI Official Synonym Symbols
cCAT; Got-1; cAspAT; AI789014
NCBI Protein Information
aspartate aminotransferase, cytoplasmic
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
Protein Family
UniProt Gene Name
Got1
UniProt Synonym Gene Names
cAspAT; cCAT
UniProt Entry Name
AATC_MOUSE
Uniprot Description
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.3; Transferase
Cellular Component: cytoplasm; cytosol; lysosome; nerve terminal; nucleus
Molecular Function: aspartate transaminase activity; carboxylic acid binding; cysteine transaminase activity; identical protein binding; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding
Biological Process: aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; dicarboxylic acid metabolic process; fatty acid homeostasis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glycerol biosynthetic process; Notch signaling pathway; oxaloacetate metabolic process
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Product Notes
The Mouse AST got1 (Catalog #AAA2709413) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709413 ELISA Kit recognizes Mouse AST. AAA Biotech's Aspartate Aminotransferase (AST) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the AST got1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartate Aminotransferase (AST), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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